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Environmental fate & pathways

Biodegradation in water: screening tests

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Description of key information

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed

Additional information

The biodegradation potential of N-benzyl-N-C16-18 (even numbered)-alkyl-N-methyl-C16-18 (even numbered)-alkyl-1-aminium chloride has been assessed through an OECD 301F conducted under GLP conditions.

A measured volume (250 ml) of inoculated mineral medium, containing 41.6 mg/L of N-benzyl-N-C16-18 (even numbered)-alkyl-N-methyl-C16-18 (even numbered)-alkyl-1-aminium chloride with a ThOD of 3.045 mgO2/mg as the sole source of organic carbon, is stirred in a closed flask at a constant temperature of 22 ± 1 °C for up to 28 days. The consumption of oxygen is determined by measuring the quantity of oxygen (produced electrolytically) required to maintain constant gas pressure in the respirometer flask. Soda lime pellets absorb evolved carbon dioxide. The amount of oxygen taken up by the test chemical, corrected for uptake by blank inoculum run in parallel, is expressed as a % of ThOD.

Under the test conditions, the % of biodegradation of N-benzyl-N-C16-18 (even numbered)-alkyl-N-methyl-C16-18 (even numbered)-alkyl-1-aminium chloride reached a maximum of 13 % at day 14, and 0 % as an artefact at the end of the 28 day testing period. This was due to an increase in blank respiration. The test item did not inhibit the microorganisms as shown by the toxicity control flask. However, N-benzyl-N-C16-18 (even numbered)-alkyl-N-methyl-C16-18 (even numbered)-alkyl-1-aminium chloride cannot be considered readily biodegradable.