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EC number: 203-921-8 | CAS number: 111-92-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- GLP guideline study; study report available
Data source
Referenceopen allclose all
- Reference Type:
- other: J-CHECK
- Title:
- Unnamed
- Year:
- 2 020
- Report date:
- 2020
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 000
- Report date:
- 2000
- Reference Type:
- other: recalculation
- Title:
- Unnamed
- Year:
- 2 020
- Report date:
- 2020
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Version / remarks:
- 1984
- GLP compliance:
- yes
Test material
- Reference substance name:
- Diethylamine
- EC Number:
- 203-716-3
- EC Name:
- Diethylamine
- Cas Number:
- 109-89-7
- Molecular formula:
- C4H11N
- IUPAC Name:
- N-ethylethanamine
- Test material form:
- liquid
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Lot number of test material: B34683L
- Purity test date: >= 99%
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: at room temperature, in the dark
- Stability under storage conditions: Infrared absorption spectrum, NMR spectrum measurement and high-performance liquid chromatograph analysis were performed on the obtained test substance, and it was confirmed that no contradiction was found with the structure of the test substance and the purity was confirmed. At the end of the test, the same measurement and analysis was performed, and when compared with the measurement and analysis results before the start of the test, no difference was found. Therefore, it was determined that the test substance was stable during storage in the sample storage in the research department.
OTHER SPECIFICS
- appearance: colorless, transparent liquid
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- At the start of exposure:
- 0.5 mL of test solution from each test container were collected and filled into a vial.
- 500 μL 2,4-Dinitrofluorobenzene (100 mmoL acetonitrile solution) and 100 μL sodium hydroxide (0.1N) were added and the solution was left at room temperature for 30 minutes or more to UV label the test substance.
- The samples were than analysed in an HPLC autosampler.
At the end of exposure:
- 1.0 - 2.0 mL of test solution from each test container were collected, centrifuged and the supernatant was filled into a vial.
- 500 μL 2,4-Dinitrofluorobenzene (100 mmoL acetonitrile solution) and 100 μL sodium hydroxide (0.1N) were added and the solution was left at room temperature for 30 minutes or more to UV label the test substance.
- The samples were than analysed in an HPLC autosampler.
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name:
- Strain: ATCC 22662
- Source (laboratory, culture collection): obtained from American Type Culture Collection
ACCLIMATION
- Acclimation period: 3 days
- Culturing media and conditions (same as test or not): same as test
- Any deformed or abnormal cells observed: no deformation or abnormal cells were observed
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 23 +-2 °C
measured temperature in the incubation chamber: 22.0 °C (0h), 22.8 °C (24 h), 23.0 °C (48 h), 23.1 °C (72 h); 22.7 °C (average) - pH:
- pH values measured in
Control: 7.4 (0h), 7.2 (72h)
Treatments: 7.4 - 10.0 (0h), 7.4 - 8.2 (72h)
For details see 'Any other information on results incl. tables' - Nominal and measured concentrations:
- nominal: 4.8, 8.6, 15.4, 27.8, 50.0 and 90.0 mg/L
measured: 0 h: 4.0, 7.4, 14.8, 26.8, 45.3 and 87.3 mg/L (83.3 to 97.0% of nominal)
72 h: 2.1, 4.1, 7.5, 13.3, 24.0 and 46.0 mg/L (43.8 to 51.1% of nominal)
Therefore the geomean concentrations were used to recalculate the ECx values (BASF SE, 2020)
Geomean concentrations: 2.9, 5.5, 10.5, 18.9, 33.0 and 63.4 mg/L - Details on test conditions:
- TEST SYSTEM
- Fill volume: 100 mL
- Aeration: no
- Initial cells density: 1*10^4 cells/ml
- Control end cells density: 194.6*10^4 cells/ml
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: standard medium (OECD medium)
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: no
- Photoperiod: 24 h
- Light intensity and quality: 4000 - 5000 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : cell density: measured daily; pH values of the test solutions: measured at the beginning and at the end of the test; temperature and light intensity: measured daily - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 34.3 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% CL: 32.0 - 36.3 mg/L
- Remarks:
- recalculated with ToxRat v3.3
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 50.9 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% CL: 49.3 - 52.4 mg/L
- Remarks:
- recalculated with ToxRat v3.3
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 11 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other:
- Remarks:
- result from JCHECK, NITE homepage; June 2019
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 54 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other:
- Remarks:
- result from JCHECK, NITE homepage, June 2019
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 50 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other:
- Remarks:
- result from study report; sectional growth rate (24 - 72h)
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 75.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% CL: 69.5 - 82.5 mg/L
- Remarks:
- result from study report; sectional growth rate (24 - 72h)
- Results with reference substance (positive control):
- - 72-h EbC50: 0.52 mg/L
Any other information on results incl. tables
The data of the original report were re-evaluated using ToxRat v3.3 in order to obtain an EC10, which is originally not reported. According to REACH Guidance document chapter R.10 Table R.10-1 (Overview of toxicity test endpoints and guidance on derivation of L(E)C50 and NOEC values) an EC10 for a long-term test which is obtained using an appropriate statistical method (usually regression analysis) will be used preferentially.
There has been a recommendation within OECD in 1996 to phase out the use of the NOEC, in particular as it can correspond to large and potentially biologically important magnitudes of effect. The advantage of regression method for the estimation of ECx is that information from the whole concentration-effect relationship is taken into account and that confidence intervals can be calculated. These methods result in an ECx, where x is a low effect percentile (e.g. 5-20%). It makes results from different experiments more comparable than NOECs. For the re-evaluation, geomean concentrations were calculated.
Result tables from original study report
Table 1: Measured concentrations of Diethylamine during a 72 -h exposure of Selenastrum capricornutum with calculated geomean concentrations (added by BASF SE, 2020)
Nominal concentration [mg/L] |
Measured concentration – 0 h (% of nominal) [mg/L] |
Measured concentration – 72 h (% of nominal) [mg/L] |
Geomean Concentration [mg/L] |
Control |
< 0.04 |
< 0.04 |
0 |
4.8 |
4.0 (83.3) |
2.1 (43.8) |
2.9 |
8.6 |
7.4 (86.0) |
4.1 (47.7) |
5.5 |
15.4 |
14.8 (96.1) |
7.5 (48.7) |
10.5 |
27.8 |
26.8 (96.4) |
13.3 (47.8) |
18.9 |
50.0 |
45.3 (90.6) |
24.0 (48.0) |
33.0 |
90.0 |
87.3 (97.0) |
46.0 (51.1) |
63.4 |
Table 2: Cell densitiy of Selenastrum capricornutum
Nominal concentration [mg/L] |
No. |
Cell Density (a) – 0 h |
Cell Density (a) – 24 h |
Cell Density (a) – 48 h |
Cell Density (a) – 72h |
Control |
1 |
1.0 |
5.8 |
38.7 |
193.3 |
|
2 |
1.0 |
5.3 |
41.8 |
198.2 |
|
3 |
1.0 |
6.1 |
39.6 |
192.4 |
|
Average |
1.0 |
5.7 |
40.0 |
194.6 |
|
S.D. |
0.0 |
0.41 |
1.59 |
3.09 |
4.8 |
1 |
1.0 |
4.9 |
34.4 |
179.7 |
|
2 |
1.0 |
4.6 |
38.2 |
190.5 |
|
3 |
1.0 |
5.5 |
39.2 |
202.8 |
|
Average |
1.0 |
5.0 |
37.3 |
191.0 |
|
S.D. |
0.0 |
0.49 |
2.52 |
11.54 |
8.6 |
1 |
1.0 |
5.1 |
36.5 |
198.6 |
|
2 |
1.0 |
5.4 |
38.2 |
189.6 |
|
3 |
1.0 |
5.7 |
37.8 |
184.4 |
|
Average |
1.0 |
5.4 |
37.5 |
190.9 |
|
S.D. |
0.0 |
0.28 |
0.86 |
7.20 |
15.4 |
1 |
1.0 |
5.4 |
36.3 |
200.0 |
|
2 |
1.0 |
5.2 |
35.4 |
188.4 |
|
3 |
1.0 |
4.9 |
35.3 |
182.4 |
|
Average |
1.0 |
5.2 |
35.7 |
190.3 |
|
S.D. |
0.0 |
0.24 |
0.55 |
8.94 |
27.8 |
1 |
1.0 |
4.7 |
39.6 |
152.2 |
|
2 |
1.0 |
5.6 |
38.7 |
147.1 |
|
3 |
1.0 |
4.9 |
37.8 |
146.1 |
|
Average |
1.0 |
5.0 |
38.7 |
148.5 |
|
S.D. |
0.0 |
0.46 |
0.91 |
3.26 |
50.0 |
1 |
1.0 |
4.1 |
28.4 |
129.4 |
|
2 |
1.0 |
3.8 |
29.9 |
132.9 |
|
3 |
1.0 |
3.8 |
30.1 |
124.4 |
|
Average |
1.0 |
3.9 |
29.5 |
128.9 |
|
S.D. |
0.0 |
0.21 |
0.89 |
4.24 |
90.0 |
1 |
1.0 |
1.5 |
3.1 |
4.0 |
|
2 |
1.0 |
1.5 |
2.6 |
2.8 |
|
3 |
1.0 |
1.9 |
3.1 |
3.8 |
|
Average |
1.0 |
1.6 |
2.9 |
3.5 |
|
S.D. |
0.0 |
0.23 |
0.30 |
0.65 |
Each value represents the mean of three sample counts
(a) Cell density x 10^4 cells/ml)
Table 3: pH values at 0 -hour and 72 -hour exposure
Nominal concentration [mg/L] |
pH – 0 h |
pH – 72 h |
Control |
7.4 |
7.2 |
4.8 |
7.4 |
7.4 |
8.6 |
7.5 |
7.5 |
15.4 |
7.7 |
7.7 |
27.8 |
8.3 |
7.8 |
50.0 |
9.4 |
8.0 |
90.0 |
10.0 |
8.2 |
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.

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