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EC number: 202-715-5 | CAS number: 98-94-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1989
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study, GLP
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
Not relevant - Analytical monitoring:
- yes
- Details on sampling:
- Sampling times: 0, 24, 48 hours
- Vehicle:
- yes
- Details on test solutions:
- A 600 mg/mL stock solution was prepared by diluting 17.67 mL of test substance to 25 mL with distilled acetone. The exposure was initiated by adding appropriate aliquots of stock solution to test vessels and adding additional dilution water to give a final volume of 200 mL.
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- - Strain: Daphnia magna straus
- Source/supplier: lab-reared
- Breeding method: illumination (2500 lux): 16 h light, temperature 20±1°C
- Age: <24 hours
- Feeding: three times weekly with algae - Selanastrum capricornutum.
- Feeding during test: no - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- Not applicable
- Hardness:
- 166 mg/L as CaC03
- Test temperature:
- 19.8 - 20.9 °C
- pH:
- 7.6-9.4
- Dissolved oxygen:
- >93% of air saturation value (8.6 mg/L)
- Salinity:
- Not applicable
- Nominal and measured concentrations:
- - Nominal concentrations (mg/L): 0 (control), 0 (solvent control), 39, 65, 108, 180, 300
- Measured concentrations (mean (mg/L)): 0, 45.0, 65, 75, 150, 241 - Details on test conditions:
- - Control group: vehicle and untreated control
STOCK AND TEST SOLUTION AND THEIR PREPARATION
- Vehicle, solvent: acetone
- Concentration of vehicle/ solvent: max. 0.12 mL/L
DILUTION WATER - Source: Lake Huron water
- Alkalinity: 54 mg/L
- TOC: 1.6 mg/L
- Ca/Mg ratio: 6/1
- Na/K ratio: 2/1
- TSS: 2.3 mg/L
- Conductance: 340 umhos/cm
TEST SYSTEM
- Exposure vessel type: 250 mL glass vessels containing 200 mL of medium
- Number of daphnids: 10/beaker, 3 beakers/treatment
- photoperiod: 16 hours
PHYSICAL MEASUREMENTS
- Measuring times: daily
TEST PARAMETER:
immobility
OBSERVATION TIMES: at 0, 24 and 48 hours
Dilution water - Daphnid dilution water is prepared by adjusting Lake Huron water, obtained from the City of Midland Water Treatment Plant before treatment, to a hardness of approximately 170 mg/L as CaC03 which is achieved after autoclaving. at 121°C and 18 psi for 35 minutes. The water is cooled and aerated before use. Daphnid dilution water is monitored weekly for pH, alkalinity, conductivity, and hardness; quarterly for selected inorganics, total suspended solids, total dissolved solids and total organic carbon (TOC); and twice annually for selected organics, pesticides and PCBs. - Reference substance (positive control):
- no
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 75 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: 95% CI 65-150 mg a.i./L
- Duration:
- 48 h
- Dose descriptor:
- LC0
- Effect conc.:
- 65.3 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 48 h
- Dose descriptor:
- LC100
- Effect conc.:
- 150 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- RESULTS:
- Effect data (Mortality): 0, 0, 0, 50, 100, 100 for concentration s of 0, 39, 65, 108, 180, 300 mg/L (nominal), respectively.
ANALYTICAL RESULTS
Linear response (r2 0.982) from 30-340 mg/L. Precision 5-26%.
The measured concentration ranged between 69-116% of nominal with a relative standard deviation of 19-48%. - Results with reference substance (positive control):
- Not applicable
- Reported statistics and error estimates:
- The acute concentration-mortality data were analysed for daily LC50 values. A computer program was used to calculate the LC50 values and corresponding 95% confidence intervals. This program has three methods available: probit analysis, moving average angle analysis and binomial probability.
- Validity criteria fulfilled:
- yes
- Conclusions:
- Three groups, each of ten Daphnia magna were exposed under static conditions to DMCHA. The results of the study were based on mean measured exposure concentrations. The 48 hour LC50 value was calculated to 75 mg/L (with 95% confidence limits of 65 to 150 mg/L). The no-observed effect concentration was observed to be 65 mg/L.
- Executive summary:
Three groups, each of ten Daphnia magna were exposed under static conditions to cyclohexyldimethylamine (DMCHA). Nominal exposure concentrations were control, control, solvent control, 38.9, 64.8, 108, 180 and 300 mg/L, mean measured concentrations were 45.0, 65.3, 74.9, 150 and 241 mg/L. The results of the study were based on mean measured exposure concentrations. After 48 hours 100% mortality was observed in the 150 and 241 mg/L treatment, there was 50% mortality at 75mg/L and no mortality in any of the other treatments or controls.
The 48 hour LC50 value was calculated to 75 mg/L (with 95% confidence limits of 65 to 150 mg/L). The no-observed effect concentration was observed to be 65 mg/L.
Reference
Analytical results
Nominal conc. (mg/L) |
Measured concentration (mg/L) |
Mean measured conc. (mg/L) |
||
0 hours |
24 hours |
48 hours |
||
Control |
nd |
nd |
nd |
- |
38.9 |
53.5 |
61.3 |
20.3 |
45.0 |
64.8 |
72.1 |
73.1 |
50.7 |
65.3 |
108 |
95.5 |
64.3 |
64.9 |
74.9 |
180 |
117.5 |
174.5 |
156.7 |
149.6 |
300 |
241.3 |
325.9 |
156.3 |
241.2 |
Daphnia mortality results
Nominal conc. (mg/L) |
Number dead Daphnia |
% mortality |
||
24 hours |
48 hours |
24 hours |
48 hours |
|
Control |
0 |
0 |
0 |
0 |
Solvent control |
0 |
0 |
0 |
0 |
45 |
0 |
0 |
0 |
0 |
65.3 |
0 |
0 |
0 |
0 |
74.9 |
15 |
15 |
50 |
50 |
150 |
30 |
30 |
100 |
100 |
241 |
30 |
30 |
100 |
100 |
Description of key information
Three groups, each of ten Daphnia magna were exposed under static conditions to cyclohexyldimethylamine (DMCHA). Nominal exposure concentrations were control, control, solvent control, 38.9, 64.8, 108, 180 and 300 mg/L, mean measured concentrations were 45.0, 65.3, 74.9, 150 and 241 mg/L. The results of the study were based on mean measured exposure concentrations. After 48 hours 100% mortality was observed in the 150 and 241 mg/L treatment, there was 50% mortality at 75mg/L and no mortality in any of the other treatments or controls.
The 48 hour LC50 value was calculated to 75 mg/L (with 95% confidence limits of 65 to 150 mg/L). The no-observed effect concentration was observed to be 65 mg/L.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 75 mg/L
Additional information
Three groups, each of ten Daphnia magna were exposed under static conditions to cyclohexyldimethylamine (DMCHA). The results of the study were based on mean measured exposure concentrations. The 48 hour LC50 value was calculated to 75 mg/L (with 95% confidence limits of 65 to 150 mg/L). The no-observed effect concentration was observed to be 65 mg/L (Mayes et al, 1989).
Additional acute aquatic toxicity data are also provided. One study from 1988 was given a Klimisch 2 as it was not a GLP compliant study. This study had a 24 -hour EC50 of 79.9 mg/L. The third study was given Klimisch 4 as there was insufficient information given in the report to determine reliability. This study had an EC50 of 75 mg/L.
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