Registration Dossier
Registration Dossier
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 206-354-4 | CAS number: 330-54-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to terrestrial plants
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to terrestrial plants: short-term
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP Guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 208 (Terrestrial Plants Test: Seedling Emergence and Seedling Growth Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Vehicle:
- yes
- Details on preparation and application of test substrate:
- The test item was applied via an acetonic stock solution (540 mg/L) in test series with concentrations of 0.013 – 1.08 mg/kg dry soil. The necessary volume of stock solution was applied on 20 g dry test soil as a vehicle. In the case of volumes < 1 mL, the stock solution was dissolved in acetone to increase the application volume. In test series with concentrations of 0.133 – 32.4 mg/kg dry soil the necessary amount of test item was transferred on a weight basis in glass beakers and dissolved in 2 mL acetone. After application on dry test soil as a vehicle, the beakers were rinsed with 2 mL acetone. This volume also was applied on the vehicle. The solvent was evaporated over night. Subsequently, the vehicle was added to the test substrate (adjusted on 40 - 60 % water holding capacity) and mixed thoroughly
The soil used in the test was a natural sandy soil (Certified RefeSol 01-A; batch IME-01, loamy sand [DIN], Org C: 0.93 %, pH 5.7, clay: 5 %) - Species:
- Allium cepa
- Plant group:
- Monocotyledonae (monocots)
- Details on test organisms:
- - Common name: onion
- Plant family: Alliaceae
- Source of seed: Carl Sperling & Co., Lüneburg, Cultivar Vaugirard
- Seed storage: The seeds were stored in the dark at the test facility at room temperature (20 ± 5 °C) and protected from moisture until use - Species:
- Triticum aestivum
- Plant group:
- Monocotyledonae (monocots)
- Details on test organisms:
- - Common name: wheat
- Plant family: Poaceae
- Source of seed: Saatgutveredelung Nückel 57392 Schmallenberg-Winkhausen, Cultivar Merlin
- Seed storage: The seeds were stored in the dark at the test facility at room temperature (20 ± 5 °C) and protected from moisture until use - Species:
- Glycine max (G. soja)
- Plant group:
- Dicotyledonae (dicots)
- Details on test organisms:
- - Common name: soybean
- Source of seed: Carl Sperling & Co., Lüneburg, Cultivar Hellfrucht
- Seed storage: The seeds were stored in the dark at the test facility at room temperature (20 ± 5 °C) and protected from moisture until use - Species:
- Lycopersicon esculentum
- Plant group:
- Dicotyledonae (dicots)
- Details on test organisms:
- - Common name: tomato
- Synonym: Solanum lycopersicum
- Plant family: Solanaceae
- Source of seed: Carl Sperling & Co., Lüneburg, Cultivar Hellfrucht
- Seed storage: The seeds were stored in the dark at the test facility at room temperature (20 ± 5 °C) and protected from moisture until use - Species:
- Beta vulgaris
- Plant group:
- Dicotyledonae (dicots)
- Details on test organisms:
- - Common name: sugar beet
- Plant family: amaranthaceae
- Source of seed: KWS Saat AG, 37555 Einbeck, Cultivar Felicita
- Seed storage: The seeds were stored in the dark at the test facility at room temperature (20 ± 5 °C) and protected from moisture until use - Species:
- Brassica napus
- Plant group:
- Dicotyledonae (dicots)
- Details on test organisms:
- - Common name: rape
- Plant family: amaranthaceae
- Source of seed: KWS Saat AG, 37555 Einbeck, Cultivar Kadore
- Seed storage: The seeds were stored in the dark at the test facility at room temperature (20 ± 5 °C) and protected from moisture until use - Test type:
- seedling emergence toxicity test
- Study type:
- laboratory study
- Substrate type:
- natural soil
- Total exposure duration:
- 14 d
- Test temperature:
- 20 ± 2 °C
- Details on test conditions:
- TEST SYSTEM
- Test container (type, material, size): Round containers of nonporous plastic with a diameter of 85 - 95 mm
- Amount of soil: ca. 280 g of moist soil
- No. of seeds per container: 5
- No. of replicates per treatment group: 4
- No. of replicates per control: 4
- No. of replicates per vehicle control: 4
GROWTH CONDITIONS
- Photoperiod: 16 hours per day
- Light source: light colour 25, universal white
- Light intensity and quality: > 7000 lux
- Day/night temperatures: 20 ± 2 °C
- Relative humidity (%): 70 ± 25 %
- Watering regime and schedules: Continuous bottom watering of the test container via glass fibre wicks.
- Any pest control method/fertilization (if used): The water was amended with fertilizer as necessary, COMPO GmbH & Co. KG, P.O. Box 2107, 48008 Münster, Germany. The concentration applied in the test was 1 mL fertilizer per liter water
EFFECT PARAMETERS MEASURED: The 14-day growth phase started when 50 % of the seedlings in the control group had emerged. This day was determined as "growth day 1". On this day, the number of emerged seedlings from all containers was recorded.
At "growth day 14", all seedlings were counted and the aboveground biomass was measured. For this, the wet mass of the plants was measured immediately after harvesting. - Nominal and measured concentrations:
- The nominal concentrations in the test containers were 0.013, 0.04, 0.12, 0.36, and 1.08 mg test item/kg (onion, tomato, sugar beet, and rape), 0.133, 0.40, 1.20, 3.60, and 10.8 mg test item/kg (wheat), and 0.40, 1.20, 3.60, 10.8, and 32.4 mg test item/kg (soy bean), respectively.
The results of HPLC-UV measurements of the highest concentrated application solution showed recovery rates of 105.6 – 110.7 % - Reference substance (positive control):
- yes
- Remarks:
- trichloroacetate
- Species:
- Allium cepa
- Duration:
- 14 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 1.08 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- seedling emergence
- Species:
- Allium cepa
- Duration:
- 14 d
- Dose descriptor:
- EC50
- Effect conc.:
- 0.35 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth
- Species:
- Allium cepa
- Duration:
- 14 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.12 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth
- Species:
- Beta vulgaris
- Duration:
- 14 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 1.08 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- seedling emergence
- Species:
- Beta vulgaris
- Duration:
- 14 d
- Dose descriptor:
- EC50
- Effect conc.:
- 0.49 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth
- Species:
- Beta vulgaris
- Duration:
- 14 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.12 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth
- Species:
- Brassica napus
- Duration:
- 14 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 1.08 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- seedling emergence
- Species:
- Brassica napus
- Duration:
- 14 d
- Dose descriptor:
- EC50
- Effect conc.:
- 0.21 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth
- Species:
- Brassica napus
- Duration:
- 14 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.12 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth
- Species:
- Glycine max (G. soja)
- Duration:
- 14 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 32.4 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- seedling emergence
- Species:
- Glycine max (G. soja)
- Duration:
- 14 d
- Dose descriptor:
- EC50
- Effect conc.:
- 1.51 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth
- Species:
- Glycine max (G. soja)
- Duration:
- 14 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.4 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth
- Species:
- Lycopersicon esculentum
- Duration:
- 14 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 1.08 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- seedling emergence
- Species:
- Lycopersicon esculentum
- Duration:
- 14 d
- Dose descriptor:
- EC50
- Effect conc.:
- 0.27 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth
- Species:
- Lycopersicon esculentum
- Duration:
- 14 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.12 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth
- Species:
- Triticum aestivum
- Duration:
- 14 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 10.8 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- seedling emergence
- Species:
- Triticum aestivum
- Duration:
- 14 d
- Dose descriptor:
- EC50
- Effect conc.:
- 0.42 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth
- Species:
- Triticum aestivum
- Duration:
- 14 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.13 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth
- Details on results:
- There was no significant effect on seedling emergence of Allium cepa (onion), Triticum aestivum (wheat), Glycine max (soy bean), Solanum lycopersicum (tomato), Beta vulgaris (sugar beet), and Brassica napus (rape) up to the highest concentration of Preventol A 6 tested.
There were statistically significant concentration related effects on growth (on a fresh weight per plant basis) of all species tested. The EC50growth for these tested species was found to be 0.21 mg (rape), 0.27 mg (tomato), 0.35 mg (onion), 0.42 mg (wheat), 0.49 mg (sugar beet), and 1.51 mg (soy bean), test item per kg soil. The NOECgrowth were 0.12 mg test item per kg soil for tomato, onion, rape and sugar beet, 0.13 mg test item per kg soil for wheat, and 0.40 mg test item per kg soil for soy bean. The biomass decrease observed in rape in the three lowest treatments is assumed to be by chance and not substance depending. Even when there are statistical differences in mean biomass, with a decrease of 5 – 9 % deviations from control showed comparable values. Therefore, the deviation up to 0.12 mg test item per kg soil is not assessed as an effect concentration. - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels:
The results of the last reference study are in agreement with experience EC50-values obtained in this institute.
Seedling emergence
Allium cepa: EC50 = 63 mg/kg
Triticum aestivum: EC50 = > 100 mg/kg
Glycine max: EC50 = > 500 mg/kg
Solanum lycopersicum: EC50 = > 500 mg/kg
Beta vulgaris: EC50 = 33 mg/kg
Brassica napus: EC50 = > 500 mg/kg
Biomass
Allium cepa: EC50 = 56 mg/kg
Triticum aestivum: EC50 = 0.8 mg/kg
Glycine max: EC50 = 0.1 mg/kg
Solanum lycopersicum: EC50 = 46 mg/kg
Beta vulgaris: EC50 = 27 mg/kg
Brassica napus: EC50 = 448 mg/kg - Reported statistics and error estimates:
- The percent inhibition of seedling emergence for each plant species was calculated as an absolute value and in comparison to the control. Survival of emerged seedlings was calculated as an absolute value. The percent inhibition of fresh weight was calculated in comparison to the control. All statistical tests (depending on normal distribution and homogeneity of variances the Fisher`s Exact Binomial Test, Williams test, Dunnett's t-test, or
Welch t-test for comparison of controls and the NOEC calculation; EC calculation) were performed with the computer software ToxRat Professional by ToxRat® Solutions GmbH. For the EC50 calculation a probit-analysis assuming log-normal distribution of the values was applied. - Executive summary:
An acute toxicity test is available for this study, in accordance with the prevailing OECD testing guidelines (208), with GLP. Six recommended plant species were used to test for seedling emergence and growth. The EC50 values for seedling emergence ranged from > 1.08 to > 32.4 mg test substance/kg. In all cases this equated to the highest concentration tested for each species and hence no significant effects of seedling emergence could be determined. The (14 d) EC50 values for seedling growth ranged from 0.21 to 1.51 mg test substance/kg. The most sensitive species used in this study was the monocot. Brassica napus (rape) with an EC50 (growth) of
0.21 mg/kg and an LC50 of 0.26 mg/kg.
Reference
Verification of test item concentration: Analytical verification (HPLC-UV) of the stock solution (coevally the application solution, nominal concentration of 540 mg/L) resulted in a mean recovery of 588.4 ± 13.0 g/L, corresponding with a recovery rate of 109.0 %. Due to the high comparability of the measured concentration with the nominal concentration, effect concentrations were determined based on nominal test concentrations
Table 1. Pathological symptoms (% plants)
TI = Test item, - = no visual symptom, a = discolouration, b = deformation, c = necrosis, d = dead plant; *: significant when compared to control (Fisher's t-test, p = 0.05, one-sided smaller)
Test species |
Solvent control |
0.013 mg TI/kg |
0.04 mg TI/kg |
0.12 mg TI/kg |
0.36 mg TI/kg |
1.08 mg TI/kg |
Allium cepa |
- |
5.6 % d |
- |
- |
100 % a |
100 % d* |
Solanum lycopersicum |
- |
10.5 % d |
- |
- |
10.5 % d |
100 % d* |
Brassica napus |
- |
- |
- |
- |
78.9 % d* |
100 % d* |
Beta vulgaris |
- |
- |
- |
- |
- |
52.6 % d* |
|
Solvent control |
0.13 mg TI/kg |
0.40 mg TI/kg |
1.20 mg TI/kg |
3.60 mg TI/kg |
10.8 mg TI/kg |
Triticum aestivum |
- |
- |
- |
- |
88.2 % a |
100 % a 52.9 % c |
|
Solvent control |
0.40 mg TI/kg |
1.20 mg TI/kg |
3.60 mg TI/kg |
10.8 mg TI/kg |
32.4 mg TI/kg |
Glycine max |
6.3 % d |
- |
- |
100 % c |
100 5c 1808 5 d |
100 % d* |
Table 2. Emergence rate at test end (%)
TI = Test item; Concentrations given as nominal concentration per kg dry mass.
Test species |
Solvent control |
0.013 mg TI/kg |
0.04 mg TI/kg |
0.12 mg TI/kg |
0.36 mg TI/kg |
1.08 mg TI/kg |
Allium cepa |
90 |
90 |
95 |
85 |
95 |
80 |
Solanum lycopersicum |
90 |
95 |
100 |
90 |
95 |
90 |
Brassica napus |
100 |
100 |
100 |
100 |
95 |
100 |
Beta vulgaris |
95 |
100 |
100 |
95 |
95 |
95 |
|
Solvent control |
0.13 mg TI/kg |
0.40 mg TI/kg |
1.20 mg TI/kg |
3.60 mg TI/kg |
10.8 mg TI/kg |
Triticum aestivum |
95 |
95 |
100 |
95 |
85 |
85 |
|
Solvent control |
0.40 mg TI/kg |
1.20 mg TI/kg |
3.60 mg TI/kg |
10.8 mg TI/kg |
32.4 mg TI/kg |
Glycine max |
80 |
70 |
85 |
80 |
80 |
75 |
Table 3. Emergence inhibition related to control at test end (%)
TI = Test item; Concentrations given as nominal concentration per kg dry mass; no significant deviation when compared to control (Fisher's t-test, p = 0.05, one-sided smaller); negative value = emergence stimulation.
Test species |
0.013 mg TI/kg |
0.04 mg TI/kg |
0.12 mg TI/kg |
0.36 mg TI/kg |
1.08 mg TI/kg |
Allium cepa |
0 |
-6 |
6 |
0 |
11 |
Solanum lycopersicum |
-6 |
-11 |
0 |
-6 |
0 |
Brassica napus |
0 |
0 |
0 |
5 |
0 |
Beta vulgaris |
-5 |
-5 |
0 |
0 |
0 |
|
0.13 mg TI/kg |
0.40 mg TI/kg |
1.20 mg TI/kg |
3.60 mg TI/kg |
10.8 mg TI/kg |
Triticum aestivum |
0 |
-5 |
0 |
11 |
11 |
|
0.40 mg TI/kg |
1.20 mg TI/kg |
3.60 mg TI/kg |
10.8 mg TI/kg |
32.4 mg TI/kg |
Glycine max |
13 |
-6 |
0 |
0 |
6 |
Table 4. Fresh weight of the shoots. Mean values ± SD (g)
TI = Test item; n.s.s. = No survived seedling; Concentrations given as nominal concentration per kg dry mass
Test species |
Solvent control |
0.013 mg TI/kg |
0.04 mg TI/kg |
0.12 mg TI/kg |
0.36 mg TI/kg |
1.08 mg TI/kg |
Allium cepa |
0.220 ±0.061 |
0.221 ±0.031 |
0.228 ±0.004 |
0.235 ±0.033 |
0.101 ±0.010 |
n.s.s. |
Solanum lycopersicum |
1.264 ±0.096 |
1.157 ±0.116 |
1.307 ±0.070 |
1.615 ±0.111 |
0.197 ±0.062 |
n.s.s. |
Brassica napus |
1.318 ±0.075 |
1.256 ±0.061 |
1.203 ±0.028 |
1.208 ±0.058 |
0.131 ±0.072 |
n.s.s. |
Beta vulgaris |
1.010 ±0.025 |
1.039 ±0.041 |
1.005 ±0.042 |
1.061 ±0.074 |
0.761 ±0.062 |
n.s.s. |
|
Solvent control |
0.13 mg TI/kg |
0.40 mg TI/kg |
1.20 mg TI/kg |
3.60 mg TI/kg |
10.8 mg TI/kg |
Triticum aestivum |
0.881 ±0.064 |
0.883 ±0.071 |
0.360 ±0.036 |
0.202 ±0.040 |
0.051 ±0.013 |
0.054 ±0.015 |
|
Solvent control |
0.40 mg TI/kg |
1.20 mg TI/kg |
3.60 mg TI/kg |
10.8 mg TI/kg |
32.4 mg TI/kg |
Glycine max |
2.141 ±0.665 |
2.512 ±0.306 |
1.008 ±0.116 |
0.491 ±0.142 |
0.416 ±0.177 |
n.s.s. |
Table 5. Growth inhibition related to control at test end (%FM)
TI = Test item; n.s.s. = No survived seedling; Concentrations given as nominal concentration per kg dry mass; *: significant when compared to control (Williams t-test, p = 0.05, one-sided smaller); negative value = growth stimulation
Test species |
0.013 mg TI/kg |
0.04 mg TI/kg |
0.12 mg TI/kg |
0.36 mg TI/kg |
1.08 mg TI/kg |
Allium cepa |
-1 |
-4 |
-7 |
54* |
n.s.s. |
Solanum lycopersicum |
9 |
-3 |
-4 |
84* |
n.s.s. |
Brassica napus |
5 |
9*# |
8*# |
90* |
n.s.s. |
Beta vulgaris |
-3 |
1 |
-5 |
25* |
n.s.s. |
|
0.13 mg TI/kg |
0.40 mg TI/kg |
1.20 mg TI/kg |
3.60 mg TI/kg |
10.8 mg TI/kg |
Triticum aestivum |
0 |
59* |
77* |
94* |
94* |
|
0.40 mg TI/kg |
1.20 mg TI/kg |
3.60 mg TI/kg |
10.8 mg TI/kg |
32.4 mg TI/kg |
Glycine max |
-17 |
53 |
77 |
81 |
n.s.s. |
#No concentration - and therefore substance - depending effects up to concentration of 0.12 mg/kg
Table 6. NOECs and EC50values for emergence (mg TI/kg DM)
NOEC = No observed effect concentration; EC = Effect concentration; CL = Confidence level; n.d. = not determined due to mathematical reasons; TI = Test item; Concentrations given as nominal concentration per kg dry mass.
Test species |
Brassica napus |
Triticum aestivum |
Solanum lycopersicum |
Allium cepa |
Beta vulgaris |
Glycine max |
NOEC |
=1.08 |
=10.8 |
=1.08 |
=1.08 |
=1.08 |
=32.4 |
EC50 (95% CL) |
> 1.08 (n.d.) |
> 10.8 (n.d.) |
> 1.08 (n.d.) |
> 1.08 (n.d.) |
> 1.08 (n.d.) |
> 32.4 (n.d.) |
Table 7. NOEC and EC values for growth inhibition on the basis of fresh mass (ECgrowth) and mortality (LC) [mg TI/kg DM]
NOEC = No observed effect concentration; EC = Effect concentration; CL = Confidence level; n.d. = not determined due to mathematical reasons; TI = test item; Concentrations given as nominal concentration per kg dry mass
Test species |
Brassica napus |
Triticum aestivum |
Solanum lycopersicum |
Allium cepa |
Beta vulgaris |
Glycine max |
NOECgrowth |
0.12 |
0.13 |
0.12 |
0.12 |
0.12 |
0.40 |
EC10growth (95% CL) |
0.11 (0.04-0.15) |
0.12 (n.d.) |
0.19 (0.17-0.21) |
0.22 (0.20-0.23) |
0.27 (0.25-0.29) |
0.33 (n.d.) |
EC50growth (95% CL) |
0.21 (0.14-0.30) |
0.42 (n.d.) |
0.27 (0.26-0.29) |
0.35 (0.34-0.35) |
0.49 (0.47-0.51) |
1.51 (n.d.) |
NOECmortality |
0.12 |
=10.8 |
0.36 |
0.36 |
0.36 |
10.8 |
LC50 (95% CL) |
0.26 (0.21-0.32) |
> 10.8 (n.d.) |
0.49 (n.d.) |
0.62 (n.d.) |
0.58 (0.28-2.01) |
10.4 (9.7-21.5) |
Description of key information
Of six recommended test species of plant the most sensitive to the test substance was the monocot. Brassica napus (rape) with an (14 d) EC50 (growth) value of 0.21 mg/kg, a NOEC (growth) of 0.12 mg/kg and a LC50 of 0.26 mg/kg. No significant effects on seedling emergence were found for any of the species tested.
Key value for chemical safety assessment
Additional information
An acute toxicity test is available for this study, in accordance with the prevailing OECD testing guidelines (208), with GLP. Six recommended plant species were used to test for seedling emergence and growth. The NOEC (14d) values for seedling emergence ranged from > 1.08 to > 32.4 mg test substance/kg. In all cases this equated to the highest concentration tested for each species and hence no significant effects of seedling emergence could be determined. The (14 d) EC50 values for seedling growth ranged from 0.21 to 1.51 mg test substance/kg and the NOEC values ranged from 0.12 mg/kg to 0.40 mg/kg. The most sensitive species used in this study was the monocot. Brassica napus (rape), with an EC50 (growth) of 0.21 mg/kg and an LC50 of 0.26 mg/kg (Simon, 2009).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.

EU Privacy Disclaimer
This website uses cookies to ensure you get the best experience on our websites.