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Diss Factsheets

Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

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Administrative data

Link to relevant study record(s)

Description of key information

In the decision letter from ECHA of 21 October 2021 (CCH-D-2114569822-39-01/F) Long-term toxicity testing on terrestrial invertebrates (OECD TG 222 or 220 or 232) or a Long-term toxicity test to terrestrial plants (OECD TG 208 with at least six species or ISO 22030) with Cyclaprop was requested. Based on the substance properties, it was decided by the lead registrant to conduct an OECD TG 222. The deadline for submission is January 26th 2023, but unfortunately, the test report will not be available before that date. Quotations from several labs were requested, but due to non-availability of some labs, it took some time to find the right lab to perform the study on the shortest notice. The OECD 222 study will be initiated on 23 February 2023 and the final results are expected to be available by the end of June 2023. The dossier will be updated as soon as possible after receiving the final reports. A letter from the test lab showing the timelines is attached to this endpoint summary.


Cyclaprop's earthworm EC10 is 45 mg/kg soil dw based on read across from Verdox, which was tested in an OECD TG 222.

Key value for chemical safety assessment

Long-term EC10, LC10 or NOEC for soil macroorganisms:
45 mg/kg soil dw

Additional information

The read across to Cyclaprop is presented in the Terrestrial toxicity Overall Endpoint summary. The executive summary of the Verdox long-earthworm study is presented below.

The executive summary of the Verdox long-term earthworm study

The long-term toxicity of Verdox to earthworm Eisenia foetida was tested in an OECD TG 222 study. The test material was dissolved in equal amounts of acetone, mixed with quartz sand and allowed to slowly evaporate and mixed with the standard soil containing 10% Sphagnum peat, 20% kaolinite clay, approximately 70% fine quartz-sand (grain size 0.05 -0.2 mm) and 0.4% calcium carbonate to adjust to pH 6.0±0.5. Adult worms were exposed to nominal concentrations in soil of 5, 15.8, 50, 158.1 and 500 mg/kg dw soil. Weights of the adult worms ranged between 322 and 596 mg. The worms were fed weekly with finely ground cattle manure. Adult worms were removed after 4 weeks of exposure, counted and weighed. The remaining offspring remained in the test containers for another four weeks. Nominal concentrations are used to present the effects. No worms survived at 500 mg/kg soil dw. The NOEC for mortality and growth was 158.1 mg/kg dw. The EC10 for reproduction was 45.1 mg/kg dw soil (95 % confidence interval is 21-65 mg/kg soil dw).