Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 932-185-7 | CAS number: 1187742-72-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 30 AUG - 02 SEP 1994
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: all concentrations at 0 and 72 h.
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution was prepared by adding 105 mg test substance to 1 L deionised water. The stock solution was diluted with dionised water to give the respective test concentrations. - Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Strain: 86.81 SAG
- Source (laboratory, culture collection): originally obtained from Institut für Wasser-, Boden- und Lufthygiene, Berlin, Germany and subsequently in-house breeding
- Age of inoculum (at test initiation): 3 days - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 24 +/- 2 °C
- pH:
- Test start: 8.4 - 8.5
Test end: 7.6 - 9.4 - Nominal and measured concentrations:
- nominal: 1, 2, 4, 8, 16 and 32 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Erlenmeyer flasks
- Initial cells density: 20000 cells/mL
- Control end cells density: 970000
- No. of vessels per concentration (replicates): 5
- No. of vessels per control (replicates): 8
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Photoperiod: continuous illumination
- Light intensity and quality: approximately 8000 Lux, white
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: photometrically at 685 nm - Reference substance (positive control):
- no
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 14 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 4.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth rate
- Reported statistics and error estimates:
- From the comparison of the areas under the growth curves, the percentage inhibition rates are calculated for each concentration.
The expect probits for each concentration were calculated by probit transformation of the percentage inhibition rates over the regression line. This probits are the basis for the following probit analysis according to Cavalli-Sforza. - Validity criteria fulfilled:
- not specified
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- (e.g. non-GLP study; no analytical verification of the test concentrations)
- Qualifier:
- according to guideline
- Guideline:
- other: German industrial standard test guideline DIN 38412, part 9
- GLP compliance:
- no
- Analytical monitoring:
- no
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 15.1 mg/L
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC20
- Effect conc.:
- 19.7 mg/L
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 32.7 mg/L
- Basis for effect:
- growth rate
Referenceopen allclose all
Table 1: Mean cell numbers
|
Cell number (x 104cells/mL) |
|||
Concentration |
Test period (h) |
|||
0 h |
24 h |
48 h |
72 h |
|
Control |
2 |
6 |
25 |
97 |
1 |
2 |
7 |
25 |
93 |
2 |
2 |
8 |
25 |
83 |
4 |
2 |
6 |
23 |
77 |
8 |
2 |
6 |
16 |
49 |
16 |
2 |
4 |
7 |
11 |
32 |
2 |
3 |
4 |
3 |
Table 2: Analytically determined test concentrations
Nominal concentration |
0 h |
48 h |
Concentration for biological valuation [mg/L] |
Control |
0 |
0 |
0 |
1 |
1.1 |
1.2 |
1 |
2 |
2.0 |
2.1 |
2 |
4 |
3.9 |
4.1 |
4 |
8 |
8.0 |
8.6 |
8 |
16 |
16.1 |
16.8 |
16 |
32 |
32.6 |
32.9 |
32 |
Stock solution |
107 |
110 |
100 |
Effect values related to nominal
concentration of the test substance.
Effect values (endpoint: biomass):
EbC10 (72 h) = 14.9 mg/L
EbC20 (72 h) = 17.9 mg/L
EbC50 (72 h) = 25.4 mg/L
pH:
Concentration [mg/L] |
uninoculated |
inoculated |
|
0 h |
72 h |
72 h |
|
Control |
8.2 |
8.12 |
9.60 |
7.812 |
8.68 |
8.06 |
9.44 |
15.625 |
8.87 |
8.09 |
9.18 |
31.25 |
9.21 |
8.18 |
8.36 |
62.5 |
9.45 |
8.33 |
8.38 |
125 |
9.69 |
8.54 |
8.75 |
250 |
9.92 |
8.80 |
8.80 |
500 |
10.24 |
9.04 |
9.03 |
62.5* |
8.25 |
8.02 |
8.50 |
500* |
8.08 |
8.00 |
8.05 |
* = neutralised samples
Inhibition may be partly due to the basic pH of the test substance:
- inhibition of the fluorescence after 72 h compared to a control:
Sample |
Inhibition [%] |
62.5 mg/L |
94.8 |
62.5 mg/L (neutralized) |
62.2 |
500 mg/L |
100.0 |
500 mg/L (neutralized) |
94.6 |
Validity according to OECDTG201 (adopted 23Mar2006):
(1) The factor of cell number measured in the control between 0 and 72 h was 42.1, which is > 16. The test fulfils this validity criterion.
(2) Evaluation of the sectional growth rates (the mean coefficient of variation of sectional growth rates calculated for the individual control replicates must not be higher than 35 %): Coefficients of variation in control replicates from 0 to 72 h were: Replicate 1: 5.9 %; Replicate 2: 7.9%; Replicate 3: 8.6 %; Replicate 4: 12.6 %. The mean of the replicate coefficients of variation in the section-by-section growth rate was:8.7%. Regarding the test period of72 h; the test fulfils this validity criterion.
(3) The coefficient of variation of average specific growth rates in control cultures was 0.7 % after 72 h, which is 7%. The test fulfils this validity criterion
Description of key information
ErC50 (72 h): 14 mg a.i./L (nominal, OECD 201)
NOErC (72 h): 2 mg a.i../L (nominal, OECD 201)
Key value for chemical safety assessment
Additional information
The toxicity of Alcohols, C12-14 (even numbered), ethoxylated (<=2.5 moles EO), sulfated, monoisopropanolamine salt to algae was tested in a GLP guideline study according to OECD 201. Green algae (Desmodesmus subspicatus) were exposed to nominal test substance concentrations of 1, 2, 4, 8, 16 and 32 mg/L for 72 h. The test concentrations were analytically verified using the MBAS assay (methylene blue active substances assay). All measured concentrations were >80% of nominal. Based on the algal growth rate an EC50 of 14 mg a.i./L was recorded. The EC10 (72 h) was 4.3 mg a.i./L.
In a study performed according to German standard DIN 38412, part 9, the toxicity of monoisopropanolamine (MIPA) to algae was investigated. The determined ErC50 was 32.7 mg/L (72-h ErC10 = 15.1 mg/L). The test was performed without neutralisation of the test concentrations.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.