3-chloro-p-toluidine

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: No OECD guideline or GLP defined.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1972

Materials and methods

Principles of method if other than guideline:

Acute oral toxicity study in male rats.

GLP compliance:

not specified

Test type:

other: Acute oral toxicity in rats

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

3-chloro-p-toluidine

EC Number:

202-446-3

EC Name:

3-chloro-p-toluidine

Cas Number:

95-74-9

Molecular formula:

C7H8ClN

IUPAC Name:

3-chloro-4-methylaniline

Details on test material:

technically pure 3-chloro-p-toluidine, dissolved in polyethylene glycol

Test animals

Species:

rat

Strain:

Wistar

Sex:

male

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

other: Lutrol

Doses:

100, 250, 500, 950, 1000, 1100, 1150, 1300 mg/kg bw.

No. of animals per sex per dose:

15

Control animals:

not specified

Results and discussion

Effect levels

Key result

Sex:

male

Dose descriptor:

LD50

Effect level:

1 053 mg/kg bw

Any other information on results incl. tables

Results:

Dose (mg/kg bw)	Toxicological result after 14 days			Signs of intoxications		Timepoint of death after days
	Nr. of death animals	Nr. of animals with symptoms	Nr. of animals used	Start	Endpoint
100	0	0	15	-	-	-
250	0	15	15	30 min	4 days	-
500	0	15	15	15 min	7 days	-
950	3	15	15	10 min	7 days	1 day
1000	3	15	15	8 min	7 days	1 -2 days
1100	9	15	15	6 min	7 days	1 -2 days
1150	13	15	15	5 min	7 days	1 -2 days
1300 days	15	15	15	4 min	-	1 day

The animals showed cyanosis, labored breathing, sedative effects and a clearly diminuated general condition after 30 min after the oral application. The signs of intoxication could be observed 4 -7 days. The timepoint of death occurred within 48 hours.

Applicant's summary and conclusion

Interpretation of results:

Category 4 based on GHS criteria

Conclusions:

The LD 50 was 1053 mg/kg bw.

Executive summary:

In an acute oral toxicity test in male Wister rats the animals were administered by gavage with the test substance dissolved in Lutrol.

The doses tested were: 100, 250, 500, 950, 1000, 1100, 1150, 1300 mg/kg bw.

The animals showed cyanosis, labored breathing, sedative effects and a clearly diminuated general condition after 30 min after the oral application. The signs of intoxication could be observed 4 -7 days. The timepoint of death occurred within 48 hours.

The LD 50 was 1053 mg/kg bw.