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EC number: 200-658-0 | CAS number: 67-52-7
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- Well performed OECD and GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 990
- Report date:
- 1990
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 E (Ready biodegradability: Modified OECD Screening Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- Barbituric acid
- EC Number:
- 200-658-0
- EC Name:
- Barbituric acid
- Cas Number:
- 67-52-7
- Molecular formula:
- C4H4N2O3
- IUPAC Name:
- pyrimidine-2,4,6(1H,3H,5H)-trione
Constituent 1
Study design
- Inoculum or test system:
- sewage, predominantly domestic (adaptation not specified)
- Details on inoculum:
- Activated sludge from laboratory sewage treatment plant
- Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 20 mg/L
- Based on:
- DOC
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- the test substance solution is treated with activated sludge (0.5 mL/900 mL) at 22 +/- 2°C in the dark.
Degradation is recorded by analysis of DOC at defiined intervals during 28 days.
Reference substance
- Reference substance:
- aniline
Results and discussion
% Degradationopen allclose all
- Parameter:
- % degradation (DOC removal)
- Value:
- 58
- Sampling time:
- 7 d
- Parameter:
- % degradation (DOC removal)
- Value:
- 95
- Sampling time:
- 14 d
- Parameter:
- % degradation (DOC removal)
- Value:
- 100
- Sampling time:
- 21 d
BOD5 / COD results
- Results with reference substance:
- 100 % biodegradation of the reference substance aniline was observed after 14 days.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- Under the conditions of the OECD Screening Test the test item is readily biodegradable. Validity of the performed study was confirmed by the result with the reference substance aniline.
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