Registration Dossier
Registration Dossier
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 308-714-7 | CAS number: 98219-47-7 A complex combination of hydrocarbons obtained by the treatment and distillation of light steam-cracked petroleum naphtha. It consists predominantly of hydrocarbons having carbon numbers predominantly in the range of C5 through C6 and boiling in the range of approximately 35°C to 80°C (95°F to 176°F).
Skin irritation / corrosion
Administrative data
- Endpoint:
- skin irritation: in vivo
- Type of information:
- migrated information: read-across based on grouping of substances (category approach)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline compliant study, GLP status unknown, limitations in reporting but otherwise acceptable.
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 991
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 404 (Acute Dermal Irritation / Corrosion)
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Benzene
- EC Number:
- 200-753-7
- EC Name:
- Benzene
- Cas Number:
- 71-43-2
- Molecular formula:
- C6H6
- IUPAC Name:
- benzene
Constituent 1
Test animals
- Species:
- rabbit
Test system
- Type of coverage:
- other: under exposure chamber
- Preparation of test site:
- shaved
- Vehicle:
- unchanged (no vehicle)
- Controls:
- not required
- Amount / concentration applied:
- Neat, 0.5 mL, to an area of 6cm2
- Duration of treatment / exposure:
- 4 hours
- Observation period:
- 144 h (i.e. 6 days) after end of application
- Number of animals:
- 6
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- erythema score
- Basis:
- mean
- Time point:
- other: 24/48/72 h
- Score:
- > 2
- Reversibility:
- no data
- Remarks on result:
- other: erythema score 3.00 at 144 h
- Irritation parameter:
- edema score
- Basis:
- mean
- Time point:
- other: 24/48/72
- Score:
- 0
- Reversibility:
- fully reversible
- Remarks on result:
- other: oedema only seen at 1 h
Any other information on results incl. tables
Mean scores (Draize et al 1944):
Time after end of application |
1 h |
24 h |
48 h |
72 h |
144 h |
Mean score for erythema |
1.00 |
2.00 |
2.20 |
2.40 |
3.00 |
Mean score for edema |
2.20 |
0.00 |
0.00 |
0.00 |
0.00 |
Applicant's summary and conclusion
- Interpretation of results:
- irritating
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- Benzene is considered to be irritating to rabbit skin (mean over 24/48/72 h for erythema >2)
- Executive summary:
The irritation potential of benzene was assessed on the skins of six shaved rabbits exposed to neat benzene for 4 hours, using an exposure chamber of 6 cm2. One hour after exposure oedema grade 2 and erythema grade 1 were documented. No oedema, but mean scores of 2.0/2.2/2.4 for erythema were recorded at 24, 48, and 72 hours after the end of exposure. Erythema increased to a mean of grade 3 at 6 days.
It is concluded that benzene is irritating to rabbit skin it is proposed that the appropriate classification Category 2, H315 be applied under GHS/CLP.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
