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Diss Factsheets

Environmental fate & pathways

Biodegradation in water and sediment: simulation tests

Administrative data

Endpoint:
biodegradation in water: sewage treatment simulation testing
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
01/28/2006 - 11/11/2006
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Well documented, GLP and conducted in accordance with OECD Guideline 301B.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2006
Report date:
2006

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: OECD 301B
Deviations:
no
Remarks:
The study was conducted according to the guideline in effect at time of study conduct.
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Reference substance name:
DBC80
IUPAC Name:
DBC80
Constituent 2
Chemical structure
Reference substance name:
-
EC Number:
481-150-8
EC Name:
-
Cas Number:
500011-86-9
Molecular formula:
C9H5BrClN3O2
IUPAC Name:
3-bromo-1-(3-chloropyridin-2-yl)-1H-pyrazole-5-carboxylic acid
Constituent 3
Reference substance name:
1H-Pyrazole-5-Carboxylic Acid, 3-Bromo-1-(3-Chloro-2-Pyridinyl)-
IUPAC Name:
1H-Pyrazole-5-Carboxylic Acid, 3-Bromo-1-(3-Chloro-2-Pyridinyl)-
Details on test material:
- Name of test material (as cited in study report): IN-DBC80
- Physical state: Tan powder
- Analytical purity: 96.7%
- Lot/batch No.: 008
- Expiration date of the lot/batch: July 22, 2008
- Storage condition of test material: Stored in the dark at room temperature.
Radiolabelling:
no

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge (adaptation not specified)
Details on inoculum:
- Source of inoculum/activated sludge: Local sewage treatment works.
- Preparation of inoculum for exposure: 4 hour aeration in laboratory.
- Pretreatment: Solids level of homogeneous sample determined by dry weight analysis.
- Initial cell/biomass concentration:Test vessels inoculated with 30 mg/L total suspended solids of inoculum.
Duration of test (contact time):
29 d
Initial test substance concentrationopen allclose all
Initial conc.:
10 other: mg C/L
Based on:
test mat.
Initial conc.:
5 other: mg C/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
CO2 evolution
Details on study design:
TEST CONDITIONS
- Volume of test solution/treatment: 3 L
- Composition of medium: Standard mineral salts test media solution.
- Additional substrate: No
- Test temperature: 20-23 degrees Celcius
- pH: 6.8-7.2
- pH adjusted: no
- Aeration of dilution water: 70-90 ml per minute, filtered through a carbon trap to remove CO2.
- Suspended solids concentration: 30 mg/L
- Continuous darkness: yes



TEST SYSTEM
- Culturing apparatus: 5 L amber-glass culture bottles, stoppered, with constant airflow by outlets connected to 3 Dreschel bottles containing 100 mL of 0.0125 M barium hydroxide each.
- Number of culture flasks/concentration: 2
- Measuring equipment: CO2 measured via titration of the barium hydroxide solutions in the Dreschel bottles.
- Test performed in closed vessels due to significant volatility of test substance: No. Closed vessels due to estimation parameter being measured.
- Details of trap for CO2 and volatile organics if used: 3 Dreschel bottles connected in series to outlets from test vessels, each containing 100 mL 0.0125 M barium hydroxide.



SAMPLING
- Sampling frequency: Day 0, 2, 4, 6, 8, 10, 12, 16, 22, 28, and 29.
- Sampling method: 20 mL barium hydroxide solution drawn from collection bottle.


CONTROL AND BLANK SYSTEM
- Inoculum blank: Mineral salts medium plus inoculum.
-Reference control- Sodium benzoate, mineral salts medium, and inoculum.
Reference substance
Reference substance:
benzoic acid, sodium salt

Results and discussion

Test performance:
Cummulative CO2 production in the control vessels after 29 days was 103 mg. This falls within the acceptable range for this method, and confirms that the inoculum was viable and the test was valid.
% Degradationopen allclose all
% Degr.:
41
Parameter:
CO2 evolution
Sampling time:
29 d
Remarks on result:
other: 10 mg C/L
% Degr.:
16
Parameter:
CO2 evolution
Sampling time:
6 d
Remarks on result:
other: 5 mg/L
% Degr.:
68
Parameter:
CO2 evolution
Sampling time:
16 d
Remarks on result:
other: 5 C/L
% Degr.:
82
Parameter:
CO2 evolution
Sampling time:
29 d
Remarks on result:
other: 5 mg C/L
Transformation products:
no
Evaporation of parent compound:
not measured
Volatile metabolites:
not measured
Residues:
not measured
Results with reference substance:
Sodium benzoate had degraded 99% in 29 days, confirming the validity of the test.

Any other information on results incl. tables

-Results for IN-DBC80 at a concentration of 10 mg C/L indicate that it is not readily biodegradable.

-Additional testing with a concentration of 5 mg C/L indicate that it is biodegradable.

Applicant's summary and conclusion

Conclusions:
Under the tested conditions, a 5 mg C/L solution of IN-DBC80 degraded 16%, 68%, and 82% in 6, 16, and 29 daysrespectivley. A 10 mg C/L solution degraded 41% in 29 days. INDBC80 would not be considered readily biodegradable by the requirements of the guideline based on the test at 10 mg C/L, however the results of the test at 5 mg C/L show that IN-DBC is biodegradable.