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Toxicological information

Basic toxicokinetics

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Administrative data

Endpoint:
basic toxicokinetics in vivo
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: scientifically acceptable and well documented

Data source

Reference
Reference Type:
publication
Title:
Metabolism of tributyl phosphate in male rats
Author:
Suzuki T, Sasaki K, Takeda M, Uchiyama M
Year:
1984
Bibliographic source:
J Agric Food Chem 32, 603-610 (1984)

Materials and methods

Objective of study:
metabolism
Principles of method if other than guideline:
The urinary metabolites of dibutyl hydrogen phosphate were examined in rats after a single intraperitoneal application 1-3 days after dose.

Test material

Constituent 1
Chemical structure
Reference substance name:
Dibutyl hydrogen phosphate
EC Number:
203-509-8
EC Name:
Dibutyl hydrogen phosphate
Cas Number:
107-66-4
Molecular formula:
C8H19O4P
IUPAC Name:
dibutyl hydrogen phosphate
Radiolabelling:
no

Test animals

Species:
rat
Strain:
Wistar
Sex:
male

Administration / exposure

Route of administration:
intraperitoneal
Vehicle:
corn oil
Remarks:
10 % solution
Duration and frequency of treatment / exposure:
single injection
Doses / concentrations
Remarks:
Doses / Concentrations:
250 mg/kg bw
No. of animals per sex per dose / concentration:
no data
Control animals:
not specified

Results and discussion

Preliminary studies:
no data
Main ADME results
Type:
metabolism
Results:
When rats were dosed with dibutyl hydrogen phosphate, much of it (47.6 %) was recovered intact in the urine.

Toxicokinetic / pharmacokinetic studies

Details on absorption:
no data
Details on distribution in tissues:
no data
Details on excretion:
no data

Metabolite characterisation studies

Metabolites identified:
yes
Details on metabolites:
no data

Any other information on results incl. tables

When rats were dosed with dibutyl hydrogen phosphate, much of it (47.6%) was recovered intact, and only small amounts of the carboxylated (0.1%) or hydroxylated (0.08%) (in the butyl side chain) derivates were formed, as well as some butyl dihydrogen phosphate (0.03%) was found.


After a single intraperitoneal dose of tributyl phosphate at 250 mg/kg bw, the major metabolite were dibutyl hydrogen phosphate, butyl dihydrogen phosphate and butyl bis(3-hydroxybutyl) phosphate.

Applicant's summary and conclusion

Conclusions:
Interpretation of results: low bioaccumulation potential based on study results
Executive summary:

Male Wistar rats (180 -210 g) were given a single intraperitoneal (ip) injection of dibutyl hydrogen phosphate dissolved 10% in corn oil at a dosage of 250 mg/kg bw. Urinary metabolites were investigated on day 1-3 after application.


When rats were dosed with dibutyl hydrogen phosphate, the majority (47.6 %) was recovered intact, and only small amounts of the carboxylated (0.1 %) or hydroxylated (0.08) (in the butyl side chain) derivates were formed, as well as some butyl dihydrogen phosphate (0.03 %) was found.


After a single intraperitoneal dose of tributyl phosphate at 250 mg/kg bw, the major metabolite were dibutyl hydrogen phosphate, butyl dihydrogen phosphate and butyl bis(3-hydroxybutyl) phosphate.