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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Specific investigations: other studies

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Administrative data

endocrine system modulation
Type of information:
experimental study
Adequacy of study:
other information
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference Type:
Structure-Oriented Research on the Antiestrogenic Effect of Organophosphate Esters and the Potential Mechanism
Li J
Bibliographic source:
Environ. Sci. Technol. 2020, 54, 14525−14534

Materials and methods

Principles of method if other than guideline:
E-screen assay in MCF-7 cells; transactivation assay in MVLN cells
Type of method:
in vitro
Endpoint addressed:
other: estrogenic and antiestrogenic effects

Test material

Constituent 1
Chemical structure
Reference substance name:
Dibutyl hydrogen phosphate
EC Number:
EC Name:
Dibutyl hydrogen phosphate
Cas Number:
Molecular formula:
dibutyl hydrogen phosphate

Results and discussion

Any other information on results incl. tables

Dibutyl phosphate (DBP) was tested for estrogenic-like and/or antiestrogenic activity in the E-screen assay (cotreated with E2) in MCF-7 cells with an EC50 value of 127 µM, compared to the positive control E2 that induced proliferation in a dose-related manner with EC50 of 11.8 nN. In the transactivation assay in MVLN cells, to investigate further estrogenic receptor activity, with single exposure and cotreatment with E2 DBP could inhibit the luciferase activities in a dose-related manner, however with EC50 of 2 mM and 9.7 mM, respectively. E2 was used as positive control, and it showed an EC50 value of 79.7 pM.
Thus, no relevant estrogenic or antiestrogenic effects became obvious for DBP.

Applicant's summary and conclusion