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Diss Factsheets

Environmental fate & pathways

Bioaccumulation: aquatic / sediment

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
bioaccumulation in aquatic species: fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1995
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Qualifier:
according to guideline
Guideline:
other: Bioaccumulation test of chemical substance in fish and shellfish", order of the prime minister, the minister of health and welfare, the minister of international trade and industry No 1, Japan, 1974
Principles of method if other than guideline:
Method: "Bioaccumulation test of chemical substance in fish and shellfish" stipulated in the Order Prescribing the Items of the Test Relating to the New Chemical Substance (1974, Order of the Prime Minister, the Minister of Health and Welfare, the MITI No. 1). This guideline corresponds to "305 C, (Bioaccumulation: Test for the Degree of Bioconcentration in Fish).
GLP compliance:
not specified
Radiolabelling:
no
Details on sampling:
- Sampling intervals/frequency for test organisms: every two weeks, n=2, control fish analysis: before the initiation and the termination of exposure, n=2
- Sampling intervals/frequency for test medium samples: twice a week, n=1
Vehicle:
not specified
Details on preparation of test solutions, spiked fish food or sediment:
It is necessary to prepare a solution of the test substance 100 times highly concentrated than that in the aquarium. If the test substance is not soluble enough in water, suitable solubilisers were used. More detailled information is not available for the substance tested .
Test organisms (species):
Cyprinus carpio
Details on test organisms:
Supplier: Sugishima fish farm
Lipid content (averange): 3.7 % (at the initiation of exposure)
Weight: about 30g, Length: about 10 cm,
Route of exposure:
aqueous
Test type:
flow-through
Water / sediment media type:
natural water: freshwater
Total exposure / uptake duration:
6 wk
Test temperature:
25+-2°C

pH:
no data
Dissolved oxygen:
6-8 mg/L

Nominal and measured concentrations:
Considering of the result of preliminary test for 48-hr LC50 and analytically detecting limit of the test substance, test concentrations were decided.
2 and 0.2 mg/L
Details on estimation of bioconcentration:
Test tank: 100L glass tank
Number of fish: 15-20 fish/Level
Renewal rate of test solutions: flow rate 200-800 mL/min
Lipid content:
4.7 %
Remarks on result:
other: end of exposure
Key result
Type:
BCF
Value:
< 0.7 dimensionless
Basis:
whole body w.w.
Time of plateau:
6 wk
Calculation basis:
steady state
Remarks on result:
other: 1 st concentration
Remarks:
Conc.in environment / dose:2 mg/l
Key result
Type:
BCF
Value:
< 7 dimensionless
Basis:
whole body w.w.
Time of plateau:
6 wk
Calculation basis:
steady state
Remarks on result:
other: 2nd concentration
Remarks:
Conc.in environment / dose:0.2 mg/l

Calculation of bioconcentration factor

BCF = concentration of test substance in fish / concentration of test substance in water

The result is reported as <0.7 - <7 at the concentration of 0.2 and 2 mg/L

LC50 48h > 250mg/L, Rice Fish

Validity criteria fulfilled:
not specified
Conclusions:
In a flow-through test, corresponding to OECD Guideline 305C a BCF of <0.7 - <7 was determined on Cyprinus carpio within 6 weeks and hence dibutyl hydrogen phosphate is not bioaccumulative.
Executive summary:

In a bioaccumulation study BCF values in the range between <0.7 and <6.9 for dibutyl hydrogen phosphate were determined with Cyprinus carpio as test organism within 6 weeks. The study was carried out at the National Institute of Technology and Evaluation, Japan following the national standard procedure "Bioaccumulation test of chemical substance in fish and shellfish", order of the prime minister, the minister of health and welfare, the minister of international trade and industry No 1, Japan, 1974; which corresponds to the OECD Guideline 305 C. Fish was exposed to dibutyl hydrogen phosphate at concentrations of 0.2 mg/l and 2 mg/l under flow-through conditions. The method of the analytical monitoring is not reported. After reaching a steady state of uptake and elimination, the remaining fish were transferred to a flow-through system containing clean water. At the concentration of 1 mg/l a BCF value of < 0.7 was determined. A BCF value of <6.9 was determined at a concentration of 0.2 mg/l. These results are in accordance with the calculated log Kow of -0.90 for the dissociated form of dibutyl hydrogen phosphate. The results show no indication of a bioaccumulation potential due to the exposure of the organisms via water.

Description of key information

Determined BCF values for dibutyl hydrogen phosphate were in the range of <0.7 - <6.9. Determinations were performed at concentrations of 0.2 and 2 mg/L. Dibutyl hydrogen phosphate is considered as not bioaccumulative.

Key value for chemical safety assessment

BCF (aquatic species):
7 dimensionless

Additional information

Should be read as <7.

In a bioaccumulation study BCF values in the range between <0.7 and <6.9 for dibutyl hydrogen phosphate were determined with Cyprinus carpio as test organism within 6 weeks. The study was carried out at the National Institute of Technology and Evaluation, Japan following the national standard procedure "Bioaccumulation test of chemical substance in fish and shellfish", order of the prime minister, the minister of health and welfare, the minister of international trade and industry No 1, Japan, 1974; which corresponds to the OECD Guideline 305 C. Fish was exposed to dibutyl hydrogen phosphateat concentrations of 0.2 mg/L and 2 mg/L under flow-through conditions. The method of the analytical monitoring is not reported. After reaching a steady state of uptake and elimination, the remaining fish were transferred to a flow-through system containing clean water. At the concentration of 1 mg/L a BCF value of < 0.7 was determined. A BCF value of < 6.9 was determined at a concentration of 0.2 mg/L. These results are in accordance with the calculated log Kow of -0.90 for the dissociated form of dibutyl hydrogen phosphate. The results show no indication of a bioaccumulation potential due to the exposure of the organisms via water.