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Diss Factsheets

Environmental fate & pathways

Biodegradation in water and sediment: simulation tests

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Administrative data

Endpoint:
biodegradation in water: sediment simulation testing
Data waiving:
study scientifically not necessary / other information available
Justification for data waiving:
other:
Cross-reference
Reason / purpose for cross-reference:
reference to other assay used for intermediate effect derivation
Reference
Endpoint:
biodegradation in water: inherent biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
05-06/1991
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Qualifier:
according to guideline
Guideline:
EU Method C.9 (Biodegradation: Zahn-Wellens Test)
Deviations:
no
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)
Principles of method if other than guideline:
Cited as Directive 87/302/EEC and DIN 38 412 part 25
GLP compliance:
yes
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, industrial, adapted
Details on inoculum:
- Activated sludge of aeration tanks of a sewage treatment plant treating predominantly sewage of industrial origin (STP Leverkusen Bürrig)
- Date of collections : 1991-05-22
- Concentration of inoculum (dry weight) : 1000 mg/L
Duration of test (contact time):
28 d
Initial conc.:
100 mg/L
Based on:
DOC
Details on study design:
The substance is dissolved in a mineral medium, inoculated with a mixed population of aquatic microorganisms and 28 days under aerobic conditions were incubated in the dark at 20 – 25°C. The biodegradation of the substance is determined during this time by the decrease of the DOC.
Parameter:
% degradation (DOC removal)
Value:
9
Sampling time:
7 d
Parameter:
% degradation (DOC removal)
Value:
97
Sampling time:
14 d
Parameter:
% degradation (DOC removal)
Value:
> 98
Sampling time:
21 d
Key result
Parameter:
% degradation (DOC removal)
Value:
> 98
Sampling time:
28 d
Validity criteria fulfilled:
yes
Interpretation of results:
inherently biodegradable
Conclusions:
Within 28 days a degradation of > 98 % was determined according to the EU Method C.9 (Biodegradation: Zahn-Wellens Test). Therefore dibutyl hydrogen phosphate is considered to be "Inherently Biodegradable".
Executive summary:

Dibutyl hydrogen phosphate is dissolved in a mineral medium, inoculated with a mixed population of aquatic microorganisms and 28 days under aerobic conditions were incubated in the dark at 20–25°C. The biodegradation of dibutyl hydrogen phosphate is determined using the removal of the DOC. Within 28 days a degradation of > 98 % was determined according to the EU Method C.9 (Biodegradation: Zahn-Wellens Test). Therefore dibutyl hydrogen phosphate is considered to be "Inherently Biodegradable".

Data source

Materials and methods

Results and discussion

Transformation products:
no

Applicant's summary and conclusion