(4-chlorophenyl)hydrazine

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP Guideline Study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1999

Report date:

1999

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes

Test material

Reference

Name:

Unnamed

Type:

Constituent

Details on test material:

- TS: CPH, BAS 500F-Stufe 100
- Purity >99 %
- Inpurity: toluene <0.5 %

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

- Preparation of stock solution: The test was performed with an aqueous extract (eluate) of the test substance.
- The test substance was stirred in M4 medium for about 20 hours at 20 +/- 2°C.
- Undissolved test substance was removed by centrifugation (approximately 20 min at about 17700 g).
- The nominal concentration of the eluate was 100 mg/L.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

- Daphnia magna Straus was originally supplied from "Institut National de Recherche Chimique appliquee, France", and has been breeded from 1978 in the Laboratories of BASF.
- 2 - 24 hours old (starting with the 3rd breed of parent animals)

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Test temperature:

20.3 - 20.9 °C

pH:

7.5 to 8.1

Dissolved oxygen:

4.9 to 8.6 mg/L

Nominal and measured concentrations:

The analytical recovery rate was after 0/48 h in all analyzed test solutions below the detection limit (< 1 mg/L). The effective concentrations mentioned here are based therefore on the nominal concentrations of the test substance. Nominal concentrations in mg/L: control, 100, 50, 25, 12.5, 6.25, 3.13, 1.56, 0.78 and 0.39

Details on test conditions:

Test conditions:
- Dilution water chemistry: A synthetic fresh water was used for culture and test purposes.
- This water was prepared on the basis of an ultrapure, demineralized water with a conductivity <0.05 uS/cm.
- For the composition of this M4 medium see ISO 10706.
- Properties of this medium:
Total hardness: 2.20 - 3.20 mmol/L
Alkalinity up to pH 4.3: 0.80 - 1.00 mmol/L
Molar ratio Ca:Mg about 4:1
pH value: 7.5 - 8.5
Conductivity: 550 - 650 uS/cm

After preparation the M4 medium is aerated tor approximately 24 h until saturation with oxygen is reached.
- Exposure vessels: test tubes (glass) with flat bottom (nominal volume 20 ml)
- Light conditions: Artificial light, type warm white, day:night rhythm = 16:8 h
- Number of Replicates: 4
- Individuals /replicate: 5

Statistics:
- EC50 values have been calculated with the moving average method.

Measurements:
- pH values and dissolved oxygen: After Oh in an additional replicate without daphnids and after 48h in replicate 1 of each tested concentration.
- Mobile daphnids: Visually after 0, 3, 6, 24 and 48 h

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

1.56 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

2.68 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

6.25 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Any other information on results incl. tables

effect values (related to nominal concentration)
-----------------------------------------------------------------------
time                  EC0                   EC50              confidence limits   EC100
(h)                     (mg/L)              (mg/L)                  95 % (mg/L)      (mg/L)
 3                        12,5	26,8	  23,0 - 31,2	    50
 6	12,5	17,7	   n.d - n.d.	    25
24	 3,13	 7,64	  6,44 - 9,07	    12,5
48	 1,56	 2,68	  2,17 - 3,31	     6,25


Mobile daphnids after x hours:
Concentration [nominal]	3h	6h	24h	48h
Control (0 mg/L)	20	20	20	20 
100 mg/l	                            0	0	0	0 
50 mg/l	                            0	0	0	0	
25 mg/l	                          12	0	0	0 
12.5 mg/l	                          20	20	0	0 
6.25 mg/l	                          20	20	16	0
3.13 mg/l	                          20	20	19	7
1.56 mg/l	                          20	20	19	18
0.78 mg/l	                          20	20	19	19
0.39 mg/l       	                          20	20	20	18