N,N'-ethylenebis(3,4,5,6-tetrabromophthalimide)

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Aug - Oct 1981

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Performed by an experienced lab using the commercial product according to guidelines in effect at the time.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1981

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: In accordance with the "Law Concerning the Examination and Regulation of Manufacture, etc., of Chemical Substances (Japan. 1973. Law No. 117.)

Principles of method if other than guideline:

14 d ready biodegradation test using high test material concentration and low innoculum concentration

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

N,N'-ethylenebis(3,4,5,6-tetrabromophthalimide)

EC Number:

251-118-6

EC Name:

N,N'-ethylenebis(3,4,5,6-tetrabromophthalimide)

Cas Number:

32588-76-4

Molecular formula:

C18H4Br8N2O4

IUPAC Name:

N,N'-ethylenebis(3,4,5,6-tetrabromophthalimide)

Test material form:

solid: crystalline

Details on test material:

light yellow crystalline powder, 99% purity, identified by infrared specturm

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Details on inoculum:

Standard activated sludge 30 ppm (w/v)

Duration of test (contact time):

14 d

Initial test substance concentration

Initial conc.:

100 other: ppm (w/v)

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation 1

Parameter followed for biodegradation estimation:

O2 consumption

Parameter followed for biodegradation estimation 2

Parameter followed for biodegradation estimation:

test mat. analysis

Remarks:

UV after extraction with THF

Details on study design:

test temperature = 25 +/- 1 degree C
Apparatus: automated closed-system oxygen conusmpitn measuring apparatus (BOD-meter)
test vessel + basal culture medium + test material (100 ppm w/v)
test vessel + basal culture medium
test vessel + water + 100 ppm test material (100 ppm w/v)
test vesel + basal culture medium + aniline (100 ppm w/v)

Reference substance

Reference substance:

aniline

Remarks:

test invalid if O2 consumption does does not exceed 40% by 7 d

Results and discussion

% Degradation

% Degradation 1

Parameter:

% degradation (O2 consumption)

Value:

0

Sampling time:

14 d

% Degradation 2

Parameter:

% degradation (test mat. analysis)

Value:

0

Sampling time:

14 d

Any other information on results incl. tables

% degradation of aniline after 7 d = 67

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

EBTBP was not readily biodegradable when tested in aerobic sludge at a high test material to sludge ratio for a duration of 14 d.

Executive summary:

EBTBP was not readily biodegradable when tested in aerobic sludge at a high test material to sludge ratio for a duration of 14 d.