2-[(2-methyl-1-oxoallyl)oxy]ethyl acetoacetate

Endpoint summary

Additional information

Toxicity to Fish:

A semi-static acute toxicity test with Rainbow trout was performed in year 2012 according to OECD Guideline 203 and GLP. The test item was not found to be acutely toxic to Rainbow trout when dispersed in water at a measured concentration of 89.1 mg/L although sub lethal effects were exhibited by the majority of the fish at a mean measured level of 89.1 mg/L. Therefore, the 96-hour LC50 could not be calculated but must be >89.1 mg/L and the no-observed effect concentration was 38.8 mg/L.

Toxicity to Daphnia magna:

A static acute toxicity test with Daphnia magna was performed in year 2012 according to OECD Guideline 202 and GLP. No immobilisation or effects on the Daphnia were noted. The 48-hour EC50 of LZ649 for the immobilisation of Daphnia magna could not be calculated but must be greater than 96.6 mg/L. The ‘no-observed effect concentration’ of LZ649 with Daphnia magna was 96.6 mg/L.

Toxicity to Algae:

A static acute toxicity test with unicellular green alga Pseudokirchneriella subcapitata was performed in year 2012 according to OECD Guideline 201 and GLP. No microscopic abnormalities of the cells were detected. After 72 hours of exposure to LZ649, the EyC50 was 60.1 mg/L. The ErC50 could not be calculated but must be greater than 69.2mg/L. Based on measured concentrations the “no observed effect concentration” (NOEC) for growth rate and yield were 11.1 mg/L. Based on measured concentrations the “lowest observed effect concentration” (LOEC) for growth rate and yield were 69.2 mg/L.

Toxicity to Microorganisms:

A static acute toxicity test with activated sludge was performed in year 2012 according to OECD Guideline 209 and GLP. The mean respirations rates of the sludge exposed to LZ649 were decreased, at most, by 27% at 1000 mg/L. Consequently the EC20/50/80 values could not be accurately determined as insufficient inhibition was observed to identify the concentration-response relationship. The lowest observed effect concentration (LOEC) for LZ649 was 1000 mg/L. The no observed effect concentration (NOEC) for LZ649 was 320 mg/L. The three-hour EC50 for 3,5-DCP (4.96 mg/L) fulfilled the validity criterion relating to sensitivity to inhibition (acceptable EC50 range 2 to 25 mg/L). The validity criterion relating to the respiration rates in the control (specific respiration rate ≥20 mgO2/gh and a coefficient of variation of≤30%) was also satisfied.

The long-term toxicity testing to Fish and Daphnia does not need to be conducted as the chemical safety assessment does not indicate a need for further investigation. The test substance did not show any toxic effects in acute toxicity studies on water organisms. No toxic findings in other acute toxicity studies (rodent) were obseved. Due to the chemical structure, the low log Pow (0.9) and the very good water solubility (18.5 g/L) of the molecule, indicates that the substance is unlikely to cross biological membranes.