Registration Dossier
Registration Dossier
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EC number: 249-020-3 | CAS number: 28443-50-7
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 996
- Report date:
- 1996
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 2-amino-5-chlorophenol
- EC Number:
- 249-020-3
- EC Name:
- 2-amino-5-chlorophenol
- Cas Number:
- 28443-50-7
- Molecular formula:
- C6H6ClNO
- IUPAC Name:
- 2-amino-5-chlorophenol
- Details on test material:
- Wet material. Assay 90.9 %; Purity > 99%
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Details on mammalian cell type (if applicable):
- TA 1537 replaced by TA97a
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9
- Test concentrations with justification for top dose:
- Minimum concentration: 0.005 mg/plate
Maximum concentration: 5.0 mg/plate
Controls
- Untreated negative controls:
- yes
- Positive controls:
- yes
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
The test substance is regarded to be not mutagenic under study conditions. - Executive summary:
No mutagenic effects have been detected.
Cytotoxic effects were observed at 5 mg/plate for all test strains with and without S9 and 1.6 mg/plate for all test strains without S9.
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