Registration Dossier
Registration Dossier
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EC number: 225-102-4 | CAS number: 4659-45-4
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: chromosome aberration
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 005
- Report date:
- 2005
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 870.5100 - Bacterial Reverse Mutation Test (August 1998)
- Qualifier:
- according to guideline
- Guideline:
- other: EC Directive 2000/32/EC, L136, Annex 4D, B.13/B.14
- Qualifier:
- according to guideline
- Guideline:
- other: Japanese Substance Control Law (JSCL) Test Guideline III. 1 Gene Mutation test with bacteria
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 2,6-dichlorobenzoyl chloride
- EC Number:
- 225-102-4
- EC Name:
- 2,6-dichlorobenzoyl chloride
- Cas Number:
- 4659-45-4
- Molecular formula:
- C7H3Cl3O
- IUPAC Name:
- 2,6-dichlorobenzoyl chloride
- Test material form:
- other: slightly yellowish liquid
- Details on test material:
- Purity: 99.7 %
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and E. coli WP2
- Test concentrations with justification for top dose:
- 10 % S9 Mix with Strain 98: (with and without metabolic activation): 50, 160, 500,1600 and 5000 µg/plate
10 % S9 Mix with the other strains: 16,50,160,500, 1600 and 5000 µg/ plate.
30% S9 Mix with the range 16-5000 µg/plate.
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Details on test system and experimental conditions:
- Experimental conditions in the incubator 37 °C.
- Evaluation criteria:
- Criteria for a valid assay: The solvent control data correspond to the laboratory´s normal control range for the spontaneous mutant frequency.
Positive controls induce increases in the muation frequency which are significant and correspond to the laboratory´s normal range.
Results and discussion
Applicant's summary and conclusion
- Conclusions:
- The findings indicate that the test substance is not mutagenic under the test conditions, either in the absence nor in the presence of an exogenous metabolic activation.
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