Registration Dossier
Registration Dossier
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EC number: 205-861-8 | CAS number: 156-62-7
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1976
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 976
- Report date:
- 1976
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- EU Method B.1 (Acute Toxicity (Oral))
- Deviations:
- not specified
- GLP compliance:
- no
- Test type:
- acute toxic class method
- Limit test:
- no
Test material
- Reference substance name:
- Calcium cyanamide
- EC Number:
- 205-861-8
- EC Name:
- Calcium cyanamide
- Cas Number:
- 156-62-7
- Molecular formula:
- CN2.Ca
- IUPAC Name:
- calcium cyanoazanediide
- Reference substance name:
- Calcium oxide
- EC Number:
- 215-138-9
- EC Name:
- Calcium oxide
- Cas Number:
- 1305-78-8
- Molecular formula:
- CaO
- IUPAC Name:
- oxocalcium
- Reference substance name:
- Carbon
- EC Number:
- 231-153-3
- EC Name:
- Carbon
- Cas Number:
- 7440-44-0
- Molecular formula:
- C
- IUPAC Name:
- carbon
- Reference substance name:
- Calcium carbonate
- EC Number:
- 207-439-9
- EC Name:
- Calcium carbonate
- Cas Number:
- 471-34-1
- Molecular formula:
- CH2O3.Ca
- IUPAC Name:
- calcium carbonate
- Reference substance name:
- Hematite (Fe2O3)
- EC Number:
- 215-275-4
- EC Name:
- Hematite (Fe2O3)
- Cas Number:
- 1317-60-8
- Molecular formula:
- Fe2O3
- IUPAC Name:
- diiron oxide
- Reference substance name:
- Urea
- EC Number:
- 200-315-5
- EC Name:
- Urea
- Cas Number:
- 57-13-6
- Molecular formula:
- CH4N2O
- IUPAC Name:
- urea
- Reference substance name:
- Silicon dioxide
- EC Number:
- 231-545-4
- EC Name:
- Silicon dioxide
- Cas Number:
- 7631-86-9
- Molecular formula:
- O2Si
- IUPAC Name:
- dioxosilane
- Reference substance name:
- Trisilicon tetranitride
- EC Number:
- 234-796-8
- EC Name:
- Trisilicon tetranitride
- Cas Number:
- 12033-89-5
- Molecular formula:
- N4Si3
- IUPAC Name:
- trisilicon tetranitride
- Reference substance name:
- Calcium dihydroxide
- EC Number:
- 215-137-3
- EC Name:
- Calcium dihydroxide
- Cas Number:
- 1305-62-0
- Molecular formula:
- CaH2O2
- IUPAC Name:
- calcium dihydroxide
- Reference substance name:
- Aluminium oxide
- EC Number:
- 215-691-6
- EC Name:
- Aluminium oxide
- Cas Number:
- 1344-28-1
- Molecular formula:
- Al2O3
- IUPAC Name:
- aluminium oxide
- Reference substance name:
- Cyanoguanidine
- EC Number:
- 207-312-8
- EC Name:
- Cyanoguanidine
- Cas Number:
- 461-58-5
- Molecular formula:
- C2H4N4
- IUPAC Name:
- 2-cyanoguanidine
- Reference substance name:
- Calcium acetylide
- EC Number:
- 200-848-3
- EC Name:
- Calcium acetylide
- Cas Number:
- 75-20-7
- Molecular formula:
- C2Ca
- IUPAC Name:
- calcium ethynediide
- Reference substance name:
- unknown
- IUPAC Name:
- unknown
- Test material form:
- solid: particulate/powder
Constituent 1
impurity 1
impurity 2
impurity 3
impurity 4
impurity 5
impurity 6
impurity 7
impurity 8
impurity 9
impurity 10
impurity 11
impurity 12
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- Young albino rats from the Institute's colony (Wistar derived) males 128 to 240 g and females 128 to 178 g weight were used. They were housed in groups of five in screen-bottomed stainless steel cages, in a well-ventilated room maintained at 25°C. Before dosing the rats were fasted overnight.
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- water
- Details on oral exposure:
- After some preliminary observations the test material was given by gavage as a 10% (w/v) aqueous suspension to groups of five males and five females in single doses of 4.80, 5.76, 8.29 or 9.95 ml per kg body weight.
- Doses:
- 480, 476, 691, 829 and 995 mg/kg bw
- No. of animals per sex per dose:
- 5
- Control animals:
- no
- Details on study design:
- After treatment the rats received stock diet and tap water ad libitum. They were observed for signs of intoxication during a 14 days period, after which autopsies were carried out on the survivors. The LD50 was calculated according to the method of Weil (Biometrics 8 (1952) 249-263).
- Statistics:
- The LD50 was calculated according to the method of Weil (Biometrics 8 (1952) 249-263).
Results and discussion
- Preliminary study:
- not indicated
Effect levelsopen allclose all
- Key result
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- ca. 765 mg/kg bw
- 95% CL:
- > 0.639 - < 0.845
- Remarks on result:
- other: calculated for calcium cyanamide 20.5%N
- Sex:
- male/female
- Dose descriptor:
- LD0
- Effect level:
- 576 mg/kg bw
- Remarks on result:
- other: calcium cyanamide technical grade (Kalkstickstoff)
- Mortality:
- Deaths occurred between 5 and 21 hours after treatment.
- Clinical signs:
- other: Within a few hours after dosing all rats showed decreased activity and humpback behaviour. Tremors in the legs and loss of conscious were frequently observed. The survivors recovered and looked quite healthy again at the end of the observation period.
- Gross pathology:
- Macroscopic examination of the survivors did not reveal treatment-related gross alterations.
- Other findings:
- no other findings
Applicant's summary and conclusion
- Interpretation of results:
- Category 4 based on GHS criteria
- Conclusions:
- From the mortality figures the LD50 of technical calcium cyanamide is calculated to be 765 mg/kg bw with 693 and 845 as the 95% confidence limits, which is equal to 639 mg/kg Calcium cyanamide, technical grade.
- Executive summary:
In an acute oral toxicity study, groups of fasted, young Wistar rats (5/sex) were given a single oral dose of calcium cyanamide, technical grade in water by gavage at doses of 480, 576, 691, 829 and 995 mg/kg bw and observed for 14 days.
Within a few hours after dosing all rats showed decreased activity and humpback behaviour. Tremors in the legs and loss of consciousness were frequently observed. Deaths occurred between 5 and 21 hours after treatment. The survivors recovered and looked quite healthy again at the end of the observation period. Macroscopic examination of the survivors did not reveal treatment-related gross alterations.
Oral LD50 = 765 mg/kg bw (693-845 mg/kg bw 95% C.I.), equal to 639 mg/kg Calcium cyanamide, technical grade.
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