1,4-Diazabicyclo[2.2.2]octane-2-methanol

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

18 - 20 Feb 2014

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

- Concentrations: Water samples were taken for quantitative analysis at the start and end of exposure (one sample was analyzed in duplicate, mean value was determined).

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: 4 L of culture water (areated before preparation) was taken and filtered through with a membrane filter (8.0 µm). 0.2 g of test substanc (adapted to purity) was weighted and diuted to 200 mL test water to prepare 1000 mg/L stock solution. 100 mL of stock solution was diluted in a 1 L flask to prepare 100 mg/L test solution
- Eluate: no
- Differential loading: yes
- Controls: Culture water without test substance

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Source: National Institute for Environmental Studies
- Lot: DM-1101
- Age at study initiation: < 24 hours
- Feeding during test: none

Test type:

static

Water media type:

freshwater

Limit test:

yes

Test temperature:

19.5 - 19.9 °C

pH:

8.11 - 8.98

Dissolved oxygen:

7.6 - 7.9 mg/L

Nominal and measured concentrations:

nominal: control, 100 mg/L
measured: < LOD, 91.3 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 100 mL glass beaker, covered with glass petri dish
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Tap water was filtered through a PF filter, thereafter through an activated charcoal filter and again through a PF filter. Measurement values (like hardness, particulate matter, pH-value, total organic carbon, etc.) were confirmed within the range of values according to 'Water Quality Criteria for Fisheries' (Japan Fisheries Resource Conservation Association, March, 2006), OECD 210 and Health and Welfare Ministry ordinance No. 101, January 28 2011
- Intervals of water quality measurement: More than once every six month

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light / 8 h dark (room light)

TEST CONCENTRATIONS
- Range finding study: yes
- Test concentrations: nominal test concentrations of 10 and 100 mg/L
- Results used to determine the conditions for the definitive study: No mortality was observed in both concentrations

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 91.3 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

- Behavioural abnormalities: No abnormal behaviour in control and test concentration.
- Mortality of control: none

Results with reference substance (positive control):

The result of the test with the standard substance (performed in February, 2014) was within the normal range in this laboratory (48 hour-EC50 = 0.57 mg/L).

Table 1: Immobilisation of Daphnia magna

Nominal (measured) concentration (mg/L)	Immobilised Daphnia (%)
	24 hours	48 hours
Control	0	0
100 (91.3)	0	0

Validity criteria fulfilled:

yes

Description of key information

LC50 (48 h): > 100 mg/L (nominal) for Daphnia magna (OECD 202)
LC50 (48 h): > 91.3 mg/L (nominal) for Daphnia magna (OECD 202)

Additional information

One experimental study investigating the short-term effects of the test substance to aquatic invertebrates is available (Tosoh Corporation, 2014). The study was performed according to OECD 202 under GLP conditions with the water flea Daphnia magna. The limit test with the test concentration of 100 mg/L (nominal) did not result in any immobilized Daphnia and no abnormal behavior was observed. Thus, EC50 values (48 h) of > 100 mg/L (nominal) and > 91.3 mg/L (measured) were derived.