Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 203-227-5 | CAS number: 104-68-7
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
Description of key information
One guideline study on freshwater green algae
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 393 mg/L
- EC10 or NOEC for freshwater algae:
- 59.7 mg/L
Additional information
The purpose of this study was to assess the potential effects of DiEPh on the growth of the freshwater green alga, Pseudokirchneriella subcapitata. Algal growth was evaluated over a 96 -hour (4 day) exposure period at nominal concentrations of 0 (medium control), 31.3, 62.5, 125, 250, 500, and 1000 mg DiEPh/L. The medium control level included six replicate vessels and the treatment concentrations included three replicate vessels per level, all inoculated at an intial nominal loading rate of 10,000 cells/ml per vessel. In addition, blank vessels were set at each level (including the control) to serve as counting blanks to correct the daily counts for potential interference of the isoton solution and/or test material, as well as to monitor pH and concentration of the test material without the algal biomass. Cell density determinations were made at approximately 24, 48, 72 and 96 hours of exposure.
Analytical verification of the test solution concentrations was performed on samples collected on days 0 (test initiation) and 4 (test termination) of the study, Measured DiEPh concentrations ranged from 95.5 to 98% of nominal on day 0 and, in replicates with algae, ranged from 91.7 to 100% of nominal on day 4. The resulting mean measured concentrations were < LLQ (medium control), 29.7, 59.7, 121, 247, 496 and 984 mg DiEPh/L
Temperatures during the exposure period ranged from 23.8 to 24 C. Light intensity for inoculated replicates ranged from 4900 lux to 5000 lux. The pH value ranged from 7.5 to 7.6 at test initiation, from 7.7 to 8.5 in pooled replicates with algae on day 3, and from 7.7 to 8.3 in pooled replicates with algae and 7.6 to 7.7 in blank replicates without algae at test termination.
The results, based on mean measured concentrations of DiEPh, were as follows:
- 72 -hour ErC50 (95% Confidence Interval) = 393 (364, 422) mg/L
- 96 -hour ErC50 (95% Confidence Interval) = 396 (355, 437) mg/L
- 72 -hour EyC50 (95% Confidence Interval) = 254 (249, 260) mg/L
- 96 -hour EyC50 (95% Confidence Interval) = 269 (266, 272) mg/L
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
