(E)-3-(benzyl(4-((4-nitrophenyl)diazenyl)phenyl)amino)propanenitrile

Water solubility

Link to relevant study record(s)

Reference

Endpoint:

water solubility

Type of information:

experimental study

Adequacy of study:

key study

Study period:

11 September 1998 to 02 November 1998

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method A.6 (Water Solubility)

Deviations:

no

GLP compliance:

yes

Type of method:

column elution method

Water solubility:

0.002 mg/L

Temp.:

20 °C

pH:

7

Details on results:

Individual Results

The coating check was successful.

According to the guideline, the data of the fractions 1 to 5 were not taken into account for the following evaluation. The following values for the water solubility were calculated from the fractions 6 to 60 for both columns.

Result run 1: Evaluation of the fractions 6 to 60. The water solubility varies between 0.0014 and 0.0022 mg/l. Average water solubility: 0.0019 mg/l (RSD: 14 %).

Result run 2: Evaluation of the fractions 6 to 60. The water solubility varies between 0.0014 and 0.0017 mg/l. Average water solubility: 0.0016mg/l (RSD: 6 %). The values for fractions 36-40 were seen as outlier and not considered.

The other fractions were not evaluated.

Column-elution method: Run 1 (applied standards: 0.04698 mg/l for fractions 6 to 30; average HPLC peak area: 0.914; 0.05020 mg/l for fractions 31 to 60; average HPLC peak area: 0.863)

Fraction No.	V (ml)	pH	HPLC peak area	cs (mg/l)
6-10	99	6-21	0.842	0.00219
11-15	98	6.31	0.675	0.00177
16-20	96	6.39	0.528	0.00141
21-25	92	6.44	0.595	0.00166
26-30	96	6.53	0.566	0.00152
31-35	97	6.57	0.736	0.00221
36-40	96	6.57	0.635	0.00192
41-45	96	6.31	0.577	0.00175
46-50	96	6.21	0.601	0.00182
51-55	96	6.20	0.714	0.00216
56-60	96	6.21	0.643	0.00195

 

Column-elution method: Run 2 (applied standards: 0.0566 mg/l for fractions 6 to 35; average HPLC peak area: 0.965; 0.05116 mg/l for fractions 36 to 60; average HPLC peak area: 0.832). *The values for fractions 36-40 were seen as outlier and not considered.

Fraction No.	V (ml)	pH	HPLC peak area	cs (mg/l)
6-10	92.0	6.42	0.518	0.00165
11-15	92.0	6.35	0.496	0.00158
16-20	92.0	6.32	0.535	0.00171
21-25	92.0	6.40	0.495	0.00158
26-30	92.0	6.31	0.499	0.00159
31-35	93.0	6.32	0.455	0.00144
36-40*	93.0	6.40	1.769	0.00585
41-45	92.0	6.33	0.453	0.00151
46-50	92.0	6.37	0.454	0.00152
51-55	92.0	6.31	0.446	0.00149
56-60	92.0	6.42	0.503	0.00168

Conclusions:

Interpretation of results (migrated information): insoluble (< 0.1 mg/L)
The water solubility cs of the test substance T-9601 (batch HI-295) at a temperature of 20 °C was determined as follows (average over both runs):
cs = 0.0017 mg/l
SD = 0.0002 mg/I
RSD = 11 %

Executive summary:

The objective of this study was the determination of the water solubility c_sof the test substance T-9601 (batch HI-295) according to guideline 92/69/EEC, appendix A.6. The study was conducted in compliance with the Principles ofGood Laboratory Practice(GLP).

 

The water solubility c_sof the test substance has been determined according to the "column-elution" method.

 

The water solubility c_s of the test substance at a temperature of 20 °C was determined as follows (average over both runs):

c_s    = 0.0017 mg/l

SD   = 0.0002 mg/I

RSD = 11 %

 

Description of key information

The water solubility c_s of the test substance has been determined to be 0.0017 mg/L.

Key value for chemical safety assessment

Water solubility:

0.002 mg/L

at the temperature of:

20 °C

Additional information

The objective of this study was the determination of the water solubility c_S of the test substance T-9601 (batch HI-295) according to guideline 92/69/EEC, appendix A.6. The study was conducted in compliance with the Principles ofGood Laboratory Practice(GLP).

The water solubility c_s of the test substance has been determined according to the "column-elution" method.