Zinc bis[O-(2-ethylhexyl)] bis[O-(isobutyl)] bis(dithiophosphate)

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non-guideline

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1981

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

GLP compliance:

yes

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Phosphorodithioic acid, mixed O,O-bis(sec-Bu and 1,3-dimethylbutyl) esters, zinc salts

EC Number:

272-238-5

EC Name:

Phosphorodithioic acid, mixed O,O-bis(sec-Bu and 1,3-dimethylbutyl) esters, zinc salts

Cas Number:

68784-31-6

Molecular formula:

C(16-24) H(36-42) O4 P2 S4 Zn

IUPAC Name:

Phosphorodithioic acid, mixed O,O-bis(sec-Bu and 1,3-dimethylbutyl) esters, zinc salts

Test animals

Species:

rabbit

Strain:

New Zealand White

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source:
- Age at study initiation: 10-12 weeks
- Weight at study initiation: 2.15-2.93 kg
- Fasting period before study:
- Housing: Housed individually in wire bottom cages
- Diet (e.g. ad libitum): approximately 115 g daily
- Water (e.g. ad libitum): ad libitum
- Acclimation period: 2 weeks


ENVIRONMENTAL CONDITIONS
- Temperature (°C): 21
- Humidity (%): 55-75
- Air changes (per hr):
- Photoperiod (hrs dark / hrs light):


IN-LIFE DATES: From: To:

Administration / exposure

Type of coverage:

occlusive

Vehicle:

unchanged (no vehicle)

Details on dermal exposure:

TEST SITE
- Area of exposure:
- % coverage: 100%
- Type of wrap if used: plastic sheet


REMOVAL OF TEST SUBSTANCE
- Washing (if done):
- Time after start of exposure: test material removed 24 hours after exposure


TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 5 g/kg bw
- Concentration (if solution):
- Constant volume or concentration used: 11.1 ml
- For solids, paste formed:


VEHICLE
- Amount(s) applied (volume or weight with unit):
- Concentration (if solution):
- Lot/batch no. (if required):
- Purity:

Duration of exposure:

24 hours

Doses:

5000 mg/kg

No. of animals per sex per dose:

5 males and 5 females

Control animals:

yes, concurrent no treatment

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: 7 and 14 days
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight, gross pathology including: nasal passages, trachea, bronchi, heart, lungs, liver, kidneys, adrenal glnds, spleen, gonads, gastrointestinal tract, bladder, body fat and skin.

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 5 000 mg/kg bw

Mortality:

None

Clinical signs:

other: Reduced food consumption and decreased motor activity.

Gross pathology:

Microscopic examination of the treated skin showed diffuse subacute dermatitis. No gross pathological changes observed at necropsy other then skin effects were evident.

Applicant's summary and conclusion

Interpretation of results:

study cannot be used for classification

Remarks:

Migrated information

Executive summary:

 

ACUTE DERMAL TOXICITY: FIVE RABBITS OF EACH SEX WERE TREATED WITH A SINGLE DERMAL APPLICATION OF 5.0 G/KG OF THE UNDILUTED TEST MATERIAL. NO DEATHS OCCURRED DURING THE STUDY. SIGNS OF TOXICITY OBSERVED WERE REDUCED FOOD CONSUMPTION AND DECREASED MOTOR ACTIVITY. THE BODY WEIGHTS OF THE TREATED MALES AND FEMALES WERE SIGNIFICANTLY LESS THAN THE CONTROLS AT 7 AND 14 DAYS. AT AUTOPSY, NO GROSS PATHOLOGICAL CHANGES OTHER THAN SKIN EFFECTS WERE OBSERVED THAT COULD BE ATTRIBUTED TO THE TEST MATERIAL.