1,2,3-Oxadiazolium, 5-amino-3-(4-morpholinyl)-, chloride (1:1)

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

biodegradation in water: screening test, other

Remarks:

Biological Oxigene demand (BOD5)

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP- and Guideline Study, minor deviations

Qualifier:

according to guideline

Guideline:

EU Method C.5 (Degradation: Biochemical Oxygen Demand)

Deviations:

yes

Remarks:

deviating from the EU guidance but in compliance to DIN 38409 H 51 only two cultures -instead of three- per concentration were measured.

Qualifier:

according to guideline

Guideline:

other: DIN 38409 H 51

GLP compliance:

yes (incl. QA statement)

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

Not adapted bacteria mix population from the domestic sewage treatment plant of Hildesheim, Germany.
3 d before test begin the dilution water (dechlorinated and autoclaved tap water with a Ca2+-Content of 2.5 mmol/l) was inoculated and nutrium (according to DIN 38409 H 51) was added.
Storage conditions: 20 +/- 1°C, protected from light, slightly aerated

Duration of test (contact time):

5 d

Initial conc.:

>= 1.25 - <= 6.25 mg/L

Based on:

test mat.

Reference substance:

other: glucose / glutamic acid p.a.

Remarks on result:

other: BOD5-Study. Results see below.

Parameter:

BOD5

Value:

0.1 g O2/g test mat.

Results with reference substance:

The results with the reference substance (202 mg O2/l) were in the validity range of 180-230 mg O2/l.

For the numerical evaluation and calculation of the BOD5 of the test substance, the mixture rule was tested. The correlation coefficient is 0.93 below the prescribed limit (> 0.995).

The difference between the measured (0.99) and the calculated oxygen consumption using the regression equation of the dilution water (0.97) is <0.1 mg / l.

The correlation coefficient shows that the series of measurements and can not be evaluated numerically. Therefore the BSB5 was estimated.

The estimated result for the test substance was:

BSB₅ = 0.1 g O₂/g Test substance

Validity criteria fulfilled:

yes

Reference 2

Endpoint:

biodegradation in water: screening test, other

Remarks:

Chemical Oxigene demand (COD)

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.6 (Degradation: Chemical Oxygen Demand)

Qualifier:

according to guideline

Guideline:

other: DIN 38409-H-41

GLP compliance:

yes

Remarks:

The test was conducted under GLP-Conditions in a testing laboratory without GLP-certification.

Oxygen conditions:

other: Not applicable for COD

Inoculum or test system:

other: Not applicable for COD

Details on study design:

according to DIN 38409 H41 / EWG 92/69 L383 A C.6

Reference substance:

other: 3 Blind test and 1 test with potassium hydrogenphthalate

Remarks on result:

other: COD-Study. Results see below.

Parameter:

COD

Value:

1.164 g O2/g test mat.

Results with reference substance:

The results with the reference substance (197 mg O2/l) were in the validity range (200 mg O2 +/- 8 mg per liter).

Validity criteria fulfilled:

yes

Reference 3

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP and Guideline Study

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

Qualifier:

according to guideline

Guideline:

EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)

GLP compliance:

yes (incl. QA statement)

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

Sludge from the STP Hildesheim (domestic)

Duration of test (contact time):

28 d

Initial conc.:

10 mg/L

Based on:

test mat.

Initial conc.:

20 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

CO2 evolution

Details on study design:

The study was conducted according to the EG-Guideline 84/449, C.5 in analogy to the OECD guideline 301 B with no deviations from the guideline.

Reference substance:

acetic acid, sodium salt

Parameter:

% degradation (CO2 evolution)

Value:

21

Sampling time:

6 d

Parameter:

% degradation (CO2 evolution)

Value:

35

Sampling time:

14 d

Parameter:

% degradation (CO2 evolution)

Value:

51

Sampling time:

21 d

Parameter:

% degradation (CO2 evolution)

Value:

65

Sampling time:

28 d

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable, but failing 10-day window

Conclusions:

The biodegradation of the substance reached the value of > 60 % degradation within 28 d but failed the 10 day criterium. Therefore it is considered for classification as not readily biodegradable.

Description of key information

Based on the results of the biodegradation test and the determination od BOD₅ and COD the substance is biodegradable but not readily biodegradable.

Key value for chemical safety assessment

Biodegradation in water:

inherently biodegradable

Type of water:

freshwater