Hexacos-1-ene

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

migrated information: read-across based on grouping of substances (category approach)

Adequacy of study:

key study

Study period:

1967-06

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: This study is classified as reliable with restriction because although the study was not GLP compliant, it was conducted according to FHSLA recommendations.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1967

Report date:

1967

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: Federal Hazardous Substances Labeling Act

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Hexadec-1-ene

EC Number:

211-105-8

EC Name:

Hexadec-1-ene

Cas Number:

629-73-2

IUPAC Name:

hexadec-1-ene

Details on test material:

- Name of test material (as cited in study report): Alpha olefins (C16)
- Substance type: C16 alpha olefin
- Physical state: Liquid
-Specific gravity at 20 degrees Celsius: 0.7817

Test animals / tissue source

Species:

other: albino rabbit

Test system

Vehicle:

not specified

Controls:

other: The non-treated eye of each animal was used as a control.

Amount / concentration applied:

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.01 millilitre

Duration of treatment / exposure:

One application/exposure

Observation period (in vivo):

Animals were observed 24, 48 and 72 hours after exposure.

Number of animals or in vitro replicates:

0.01 ml of the test material was instilled into one eye of six male rabbits.

Details on study design:

SCORING SYSTEM: The Draize scoring system was used.

Results and discussion

In vivo

Results

Irritation parameter:

other: Draize

Basis:

mean

Time point:

other: 24 hour

Score:

0.7

Remarks on result:

other: Not irritating

Irritant / corrosive response data:

Instillation of 1-hexadecene in the eyes produced mild, transient redness of the conjunctival tissue. No effects on the cornea or iris were observed. Based on this test result, the author concluded that 1-hexadecene is not an eye irritant to albino rabbits.

Other effects:

The eye treated with control substance, 5% Ivory solution, showed greater response than any of the test substances.

Applicant's summary and conclusion

Interpretation of results:

other: Not irritating according to EU CLP classification

Remarks:

Criteria used for interpretation of results: EU

Conclusions:

Instillation of 1-hexadecene in the eyes produced mild, transient redness of the conjunctival tissue. No effects on the cornea or iris were observed. Based on this test result, the author concluded that 1-hexadecene is not an eye irritant to albino rabbits.

Executive summary:

In a primary eye irritation study, 0.1 millilitre of 1-hexadecene was instilled into the conjunctival sac of six male albino rabbits. Treated eyes were not washed during exposure. The animals were observed at 24, 48 and 72 hours after exposure. Irritation was scored by the Draize method. In this study, 1-hexadecene is not an eye irritant based on the 24 hour average redness Draize score of 0.7.

This study received a Klimisch score of 2 and is classified as reliable with restrictions because although the study was not GLP compliant, it was conducted according to FHSLA recommendations.