1-Butanaminium, 4-hydroxy-N,N,N-trimethyl-4-oxo-, inner salt

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2000

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: study performed under GLP and in accordance with Czechian testing methods (similar to OECD-/EU-testing guidelines)

Cross-reference

Reason / purpose for cross-reference:

reference to same study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2000

Report date:

2000

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: Method III (Growth inhibition of algae), Regulation of the Ministry of Environment of the Czech Republic No. 299/1998, Annex 2 (identical with Method C.3 Algal inhibition test Directive 67/548/EEC)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Details on test material:

- Name of test material (as cited in study report): 4-Trimethylaminobutyrobetain
- Molecular formula: C7H1SN02
- Physical state: liquid
- Stability under test conditions: yes
- Storage condition of test material: at room temperature

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

No auxiliary solvent used; solution was tested as such: The test item solution was further diluted with water to prepare the corresponding test concentrations. In the pretest, doses of 0, 200, 400, 600, 800 and 1000 mg/l were tested. As no growth inhibition was observed, the main test was performed with a limit concentration of 1000 mg/l nominal.

Test organisms

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

TEST ORGANISM
- Common name: Scenedesmus subspicatus Brinkmann
- Strain: 1953/SAG 86
- Source (laboratory, culture collection): Botanical Institute (Botanického Ústavu AV) ČR Průhonice delivered 14. June 2000
- Method of cultivation: with agar in 100 ml flasks at room temperature and artificial light

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

72 h

Test conditions

Hardness:

not indicated

Test temperature:

22.5°C

pH:

The pH ranged from 7.83 to 8.08 during the study

Dissolved oxygen:

not indicated

Salinity:

Freshwater was used

Nominal and measured concentrations:

A range-finding test with 0, 200, 400, 600 , 800 and 1000 mg/l was performed; due to the lack of effects, the main test was performed with a limit concentration of 1000 mg/l

Details on test conditions:

GROWTH MEDIUM
- Standard medium used: yes

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 72 hours
- Light intensity and quality: min. 6000 - max. 10000 Lux

TEST CONCENTRATIONS
- Range finding study: yes
- Test concentrations: 0, 200, 400, 600, 800 and 1000 mg/l in pretest; 1000 mg/l in main test
- Results used to determine the conditions for the definitive study: yes; no effects observed in pretest

Reference substance (positive control):

yes

Remarks:

K2Cr207

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Basis for effect:

biomass

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Basis for effect:

growth rate

Results with reference substance (positive control):

EbC50 (0-72h) = 0,54 mg/I
ErC50 (0-72h) = 1,16 mg/l

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The ErC50 and EbC50 (0-72 h) were determined to be > 100 mg/l.

Executive summary:

The test was performed in 2000 as limit test under GLP. A nominal concentration of 1000 mg/l was tested