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Acute Fish Toxicity:

Water accommodated fractions of hydrocarbons, C10-C12, isoalkanes, <2% aromatics produced a 96-hour LL50 value of >1000 mg/L and LL0 value of 1000 mg/L with rainbow trout, Oncorhynchus mykiss. No mortality was observed in either the control or treatment test systems. These data are used as read-across data to hydrocarbons, C11-C12, isoalkanes, <2% aromatics.

Chronic Fish Toxicity:

The hydrocarbons, C11-C12, isoalkanes, <2% aromatics, estimated freshwater fish 28-day NOELR (No Observed Effect Loading Rate) value is 0.209 mg/l based on growth.

Acute Invertebrate Toxicity:

Freshwater:

Water accommodated fractions of hydrocarbons, C10-C12,isoalkanes, <2% aromatics, did not produce a 50% effect (immobility) with Daphnia magna at a loading of 1000 mg/L after a 48-hour exposure. Therefore, the 48-hour LL50 is reported as >1000 mg/L. There was no mortality at the 1000 mg/L loading level after 48 hours. Therefore, the 48-hour LL0 for mortality is reported as 1000 mg/L. There was also no mortality in the control. These data are used as read-across data to hydrocarbons, C11-C12, isoalkanes, <2% aromatics.

Marine:

Water accommodated fractions of hydrocarbons, C10-C12,isoalkanes, <2% aromatics, did not produce a 50% effect (mortality) with Chaetogammarus marinus at loadings up to 1000 mg/L after a 96-hour exposure. Therefore, the 96-hour LL50 is reported as >1000 mg/L. There was no mortality at the 1000 mg/L loading level after 96 hours. Therefore, the 96-hour NOELR for mortality is reported as 1000 mg/L. There was also no mortality in the control. These data are used as read-across data to hydrocarbons, C11-C12, isoalkanes, <2% aromatics.

Chronic Invertebrate Toxicity:

Hydrocarbons, C11 -C12, isoalkanes, <2% aromatics, showed no effects on survival, reproduction, or length at nominal and measured concentrations of 0.020 and 0.011mg/L, respectively. Therefore, the 21-Day No Observed Effect Loading Rate (NOELR) and No Observed Effect Concentration (NOEC) values are 0.020 and 0.011 mg/L, respectively, for these endpoints based upon nominal loading rates and measured (geometric means) concentrations.

Algal Toxicity:

Growth of alga cultures, as measured by biomass and growth rate, exposed to a water accommodated fraction of hydrocarbons, C10-C12, isoalkanes, <2% aromatics, was not inhibited over 72 hours. The growth of algae in the the test systems was greater than the controls, which performed normally. Therefore, the 72-hr EL50 values for the two endpoints are reported as >1000 mg/L. The 72-hr NOELR values for biomass and growth rate are reported as 1000 mg/L, respectively. These data are used as read-across data to hydrocarbons, C11-C12, isoalkanes, <2% aromatics.

Toxicity to microorganisms:

Isododecane at the highest loading rate, 2 ml/L, did not inhibit the respiration rate of Pseudomonas putida over an exposure period of 5 hours. These data are used as read-across data to hydrocarbons, C11-C12, isoalkanes, <2% aromatics. In addition, the hydrocarbons, C11-C12, isoalkanes, <2% aromatics, estimated protozoan, Tetrahymena pyriformis, 48-hr EL50 value for this substance is >1000 mg/L based on growth inhibition.