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Ecotoxicological information

Long-term toxicity to fish

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Endpoint:
long-term toxicity to fish, other
Type of information:
other: EU Risk Assessment
Adequacy of study:
other information
Reliability:
other: EU Risk Assessment
Rationale for reliability incl. deficiencies:
other: no reliability is given as this is a summary entry for the EU RAR
Principles of method if other than guideline:
EU Risk Assessment
GLP compliance:
not specified

EU Risk Assessment (2003):


 


Long-term studies on fish are all based on measured concentrations. The reported values for NOEC are close together. They range between 0.04 mg/l (Broecker et al., 1984) and 0.50 mg/l (Carlson and Kosian, 1987). All in EU RAR cited studies are considered to be valid.


 


The study on Zebrafish (Brachydanio rerio) was performed according to a UBA guideline


(1982) in a dynamic system. The fish were 4 to 7 months old. The aquarium contained 36 l and the flow was 1.2 l/h. The concentrations were measured. LOEC was 0.18 mg/l (20% mortality and several behavioural abnormalities) (Broecker et al., 1984).


 


Suter and Rosen (1988) report the chronic MATC value to the marine fish Cyprinodon variegatus to be 0.222 mg/l. NOEC is estimated to be approximately half this value.


 


The lowest NOEC in long-term studies was 0.04 mg/l (mortality and behaviour, Zebra fish).

Executive summary:

EU Risk Assessment, 2003:


 


Long-term studies on fish are all based on measured concentrations. The reported values for NOEC are close together. They range between 0.04 mg/l (Broecker et al., 1984) and 0.50 mg/l (Carlson and Kosian, 1987). All in EU RAR cited studies are considered to be valid.


The study on Zebrafish (Brachydanio rerio) was performed according to a UBA guideline (1982) in a dynamic system. The fish were 4 to 7 months old. The aquarium contained 36 l and the flow was 1.2 l/h. The concentrations were measured. LOEC was 0.18 mg/l (20% mortality and several behavioural abnormalities) (Broecker et al., 1984).


Suter and Rosen (1988) report the chronic MATC value to the marine fish Cyprinodon variegatus to be 0.222 mg/l. NOEC is estimated to be approximately half this value.


The lowest NOEC in long-term studies was 0.04 mg/l (mortality and behaviour, Zebra fish).

Endpoint:
long-term toxicity to fish, other
Type of information:
other: BUA report
Adequacy of study:
other information
Reliability:
other: BUA report
Rationale for reliability incl. deficiencies:
other: no reliability is given as this is a summary entry for the BUA report
Principles of method if other than guideline:
BUA report
GLP compliance:
not specified

BUA report (1987):


 


Extended fish tests (14 d) gave an NOEC of 0.04 mg/l (Broecker et al., 1984).


The BUA report doesn't state NOEC values for other above cited studies.

Executive summary:

BUA report, 1987:


 


Extended fish tests (14 d) gave an NOEC of 0.04 mg/l (Broecker et al., 1984).


The BUA report doesn't state NOEC values for other above cited studies.

Endpoint:
long-term toxicity to fish, other
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Original reference not available.
GLP compliance:
not specified
Test organisms (species):
Jordanella floridae
Executive summary:

Smith, 1991:


egg hatchability:      1.43 mg/L
10d larval survival:   1.13 mg/L
lowest chronic level:  1.13 mg/L
dilution water: dechlorinated Lake Superior water 25 +/- 2 °C




Endpoint:
fish early-life stage toxicity
Type of information:
experimental study
Adequacy of study:
other information
Study period:
1995
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
abstract
Remarks:
GLP-Guideline study, only results are published, study report not available
Qualifier:
according to guideline
Guideline:
OECD Guideline 204 (Fish, Prolonged Toxicity Test: 14-day Study)
Deviations:
not specified
Qualifier:
according to guideline
Guideline:
OECD Guideline 210 (Fish, Early-Life Stage Toxicity Test)
Deviations:
not specified
GLP compliance:
yes
Analytical monitoring:
not specified
Vehicle:
not specified
Test organisms (species):
Oryzias latipes
Water media type:
freshwater
Limit test:
no
Duration:
14 d
Dose descriptor:
LC50
Effect conc.:
1.6 mg/L
Remarks on result:
other: OECD TG 204
Duration:
21 d
Dose descriptor:
LC50
Effect conc.:
1.5 mg/L
Remarks on result:
other: OECD TG 210
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
0.26 mg/L
Remarks on result:
other: OECD TG 210
Executive summary:

NITE,1995:


The toxicity of 1,2,4-TCB to Oryzias latipes in fresh water was investigated according to OECD guideline 204 and 210:


14 d; LC50 = 1.6 mg/L (OECD 204)


21 d; LC50 = 1.5 mg/L (OECD 210)


21 d; NOEC = 0.26 mg/L (OECD 210)


 

Endpoint:
adult fish: sub(lethal) effects
Type of information:
experimental study
Adequacy of study:
other information
Study period:
1984
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: GLP-Guideline study. Test procedure according to national standards.
Qualifier:
according to guideline
Guideline:
other: Prüfrichtlinie I 4.5 - 97125-2/6, Umweltbundesamt, 06.05.1982
GLP compliance:
yes
Analytical monitoring:
yes
Vehicle:
no
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Test type:
flow-through
Water media type:
freshwater
Limit test:
no
Total exposure duration:
21 d
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
0.04 mg/L
Nominal / measured:
meas. (not specified)
Duration:
21 d
Dose descriptor:
other: LC20
Effect conc.:
0.18 mg/L
Nominal / measured:
meas. (not specified)
Duration:
21 d
Dose descriptor:
other: LC20
Effect conc.:
0.7 mg/L
Nominal / measured:
meas. (not specified)
Duration:
21 d
Dose descriptor:
other: LC100
Effect conc.:
1.9 mg/L
Nominal / measured:
meas. (not specified)

- depending up on test concentration fishes showed behaviour modifications


- some of them were: swimming on water surface and bottom of tank, accelerated respiration, trembling, apathy 

Executive summary:

Markert, 1984:


The toxicity of 1,2,4-TBC to Danio rerio (Brachydanio rerio) was investigated in a 21 d study according to test guideline 4.5 - 97125-2/6, Federal Environmental Agency, 06.05.1982. The 21d-NOEC value was determined to be 0.04 mg/L.


 

Endpoint:
long-term toxicity to fish, other
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Original reference not available.
GLP compliance:
not specified
Analytical monitoring:
yes
Test organisms (species):
Pimephales promelas
Duration:
8 d
Dose descriptor:
LC50
Effect conc.:
1.8 mg/L
Executive summary:

Hall, 1984:


The toxicity of 1,2,4-TCB to Pimephales promelas was investigated in an 8-day study. The LC50 value was determined as 1.8 mg/L and analytically monitored by GC.

Endpoint:
long-term toxicity to fish, other
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Original reference not available.
GLP compliance:
no
Test organisms (species):
Pimephales promelas
Duration:
32 d
Dose descriptor:
NOEC
Effect conc.:
0.29 mg/L
Executive summary:

Atri, 1986:


The toxicity of 1,2,4-TCB to Pimephales promelas was investigated in an 32-day study. The NOEC value was determined as 0.29 mg/L. 

Endpoint:
long-term toxicity to fish, other
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Original reference not available.
GLP compliance:
not specified
Analytical monitoring:
yes
Test organisms (species):
Pimephales promelas
Duration:
32 d
Dose descriptor:
other: EC0
Effect conc.:
0.5 - 1 mg/L
Executive summary:

Ahmad, 1984:


The toxicity of 1,2,4-TCB to Pimephales promelas was investigated in an 32-day study. The LC0 value was determined as 0.5 mg/L and analytically monitored by GC.

Description of key information

For transported isolated intermediates according to REACh, Article 18, this endpoint is not a data requirement. However, data is available for this endpoint and is thus reported under the guidance of "all available data".


 


Generally, following values have been reported for the long-term toxicity to fish:


NOEC: 0.04 - 0.5 mg/L


LOEC: 0.18 mg/L 


The values were obtained with respect to different guidelines and exposure times.


 


EU Risk Assessment, 2003:
Long-term studies on fish are all based on measured concentrations. The reported values for NOEC are close together. They range between 0.04 mg/l (Broecker et al., 1984) and 0.50 mg/l (Carlson and Kosian, 1987). All in EU RAR cited studies are considered to be valid.
The study on Zebrafish (Brachydanio rerio) was performed according to a UBA guideline (1982) in a dynamic system. The fish were 4 to 7 months old. The aquarium contained 36 l and the flow was 1.2 l/h. The concentrations were measured. LOEC was 0.18 mg/l (20% mortality and several behavioural abnormalities) (Broecker et al., 1984).
Suter and Rosen (1988) report the chronic MATC value to the marine fish Cyprinodon variegatus to be 0.222 mg/l. NOEC is estimated to be approximately half this value. The lowest NOEC in long-term studies was 0.04 mg/l (mortality and behaviour, Zebra fish).


BUA report, 1987:
Extended fish tests (14 d) gave an NOEC of 0.04 mg/l (Broecker et al., 1984).
The BUA report doesn't state NOEC values for other above cited studies.


Smith, 1991:
egg hatchability: 1430 µg/l
10d larval survival: 1130 µg/l
lowest chronic level: 1130 µg/l
dilution water: dechlorinated Lake Superior water 25 +/- 2 °C



Hall, 1984:
The toxicity of 1,2,4-TCB to Pimephales promelas was investigated in an 8-day study. The LC50 value was determined as 1.8 mg/L and analytically monitored by GC.


Atri, 1986:
The toxicity of 1,2,4-TCB to Pimephales promelas was investigated in an 32-day study. The NOEC value was determined as 0.29 mg/L.


Ahmad, 1984:
The toxicity of 1,2,4-TCB to Pimephales promelas was investigated in an 32-day study. The LC0 value was determined as 0.5 mg/L and analytically monitored by GC.



NITE,1995:
The toxicity of 1,2,4-TCB to Oryzias latipes in fresh water was investigated according to OECD guideline 204 and 210:
14 d; LC50 = 1.6 mg/L (OECD 204)
21 d; LC50 = 1.5 mg/L (OECD 210)
21 d; NOEC = 0.26 mg/L (OECD 210)


Markert, 1984:
The toxicity of 1,2,4-TBC to Danio rerio (Brachydanio rerio) was investigated in a 21 d study according to test guideline 4.5 - 97125-2/6, Federal Environmental Agency, 06.05.1982. The 21d-NOEC value was determined to be 0.04 mg/L.


 

Key value for chemical safety assessment

Additional information