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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Link to relevant study record(s)

Description of key information

No toxicity observed even at highest concentration level tested.
RA_CAS 67-48-1_Scenedesmus subspicatus_DIN 38412 L9: ErC10(72h) > 500 mg/L, EbC10(72h) > 500 mg/L, ErC50(72h) > 500 mg/L, EbC50(72h) > 500 mg/L, ErC10(96h) > 500 mg/L, EbC10(96h) > 500 mg/L, ErC50(96h) > 500 mg/L, EbC50(96h) > 500 mg/L

Key value for chemical safety assessment

EC50 for freshwater algae:
500 mg/L
EC10 or NOEC for freshwater algae:
500 mg/L

Additional information

Toxicity to aquatic algae was not investigated with the target substance Choline hydrogen carbonate. A valid experimental result is available for the reliable read-across substance Choline chloride (CAS 67-48-1). Both substances belong to the group of quaternary amine salts (more specific: choline salts, i.e. (2-hydroxyethyl)trimethylammonium salts), which will dissociate immediately in aqueous environment. Choline and its metabolites are important for different functions in the body of animate beings and thus a naturally occurring compound. Thus, the substance characteristics of both substances are comparable. For the detailed justification of this procedure, please refer to the separate read-across statement by Chemservice S.A. (2013).

Scenedesmus subspicatus (new name: Desmodesmus subspicatus) was used in an experiment according to the German guideline DIN 38412 L9 in order to test the toxicity of Choline chloride towards freshwater algae (BASF AG, 1989). Test concentrations were chosen as followed: 0 (control), 10, 25, 50, 100, 250 and 500 mg/L.

Potassium dichromate served as reference substance. Samples were taken and evaluated after 72 and 96h. A fluorometric determination of the cell count was performed every 24 h. As results, the EC50(72h) and EC50(96h) resulted in values > 500 mg/L based on growth rate as well as on biomass, respectively. The same applies to the determined EC10 values after 72 h and 96 h.