Registration Dossier
Registration Dossier
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EC number: 201-368-7 | CAS number: 81-64-1
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 2 000
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- JAPAN: Guidelines for Screening Mutagenicity Testing Of Chemicals
- Principles of method if other than guideline:
- The mutagenic potential of quinizarine was examined in the Salmonella/microsome test. Bacteria of 4 histidine auxotrophic Salmonella typhimurium strains (TA 98, TA 100, TA 1535, and TA 1537) and Escherichia coli WP2uvrA were exposed to doses up to 5000 µg per plate.
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 1,4-dihydroxyanthraquinone
- EC Number:
- 201-368-7
- EC Name:
- 1,4-dihydroxyanthraquinone
- Cas Number:
- 81-64-1
- Molecular formula:
- C14H8O4
- IUPAC Name:
- 1,4-dihydroxy-9,10-dihydroanthracene-9,10-dione
- Details on test material:
- purity: 97.3%
source: Tokyo Kasei Kogyo co., Ltd.
Constituent 1
Method
- Target gene:
- no data
Species / strain
- Species / strain / cell type:
- other: S. typhimurium TA 1535, TA 1537, TA 98 and TA 100 and Escherichia coli WP2uvrA
- Additional strain / cell type characteristics:
- not specified
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 mix
- Test concentrations with justification for top dose:
- 0, 1.22, 4.88, 19.5, 78.1, 156, 313, 625, 1250, 2500 or 5000 µg/plate
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- not specified
- Positive control substance:
- 9-aminoacridine
- sodium azide
- other: 2-(2-furyl)-3-(5-nitro-2-furyl)acrylamide, 2-aminoanthracene
Results and discussion
Test resultsopen allclose all
- Key result
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- positive
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Additional information on results:
- No data.
- Remarks on result:
- other: Quinizarine was positive in this Ames test on S. typhimurium strain TA 1537 with and without metabolic activation, whereas quinizarine was negative in S. typhimurium strains TA 98, TA100, TA1535 and Eschrichia coli stain WP2uvrA.
Any other information on results incl. tables
Quinizarine (1,4-dihydroxyanthraquinone) was positive in this Ames test on S. typhimurium strain TA 1537 with and without metabolic activation, wheras in quinizarine was negative in S. typhimurium strains TA 98, TA100, TA1535 and Eschrichia coli stain WP2uvrA.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results: positive
- Executive summary:
The mutagenic potential of quinizarine was examined in the Salmonella/microsome test. Bacteria of 4 histidine auxotrophic Salmonella typhimurium strains (TA 98, TA 100, TA 1535, and TA 1537) and Escherichia coli WP2uvrA were exposed to doses up to 5000 µg per plate.
Quinizarine was positive in this Ames test on S. typhimurium strain TA 1537 with and without metabolic activation, whereas quinizarine was negative in S. typhimurium strains TA 98, TA100, TA1535 and Eschrichia coli stain WP2uvrA.
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