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Environmental fate & pathways

Biodegradation in water: screening tests

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Description of key information

Within 28 days a degradation rate of 7 % was determined. The test item is considered to be "Not Readily Biodegradable".

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed

Additional information

To test for its ready biodegradability potential, the substance was incubated for 28 days in continuously stirred 250 ml closed flask (two replicates) in the dark with an inoculum of mixed population of aquatic microorganisms (activated sludge) from domestic wastewater treatment plants. In this assay, biodegradation was estimated by biological oxygen demand (BOD) over time. BOD was determined daily measuring the drop in pressure in the automated respirometer flasks. The incubation temperature was 22 ± 1 °C, pH was 7.3 - 8.9. The concentration of innoculum was 30 mg /L and the one of test substance was 100 mg/L. Degradation was calculated by substracting the amount BOD in the negative (innoculum only) control from that in the test material or positive control at any given time point and divided by the thereotical oxygen demand (ThOD). The 28-day degradation was 7 % and for the positive control (with reference substance benzoic acid, sodium salt) the biodegrdation was 86 % after 14 days. In conclusion 1,4-dihydroxyanthraquinone is not readily biodegradable.