2-[(p-aminophenyl)sulphonyl]ethyl hydrogensulphate

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

10. Feb 1988 - 11 Mar 1988

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

2-[(p-aminophenyl)sulphonyl]ethyl hydrogensulphate

EC Number:

219-669-7

EC Name:

2-[(p-aminophenyl)sulphonyl]ethyl hydrogensulphate

Cas Number:

2494-89-5

Molecular formula:

C8H11NO6S2

IUPAC Name:

[2-(4-aminobenzenesulfonyl)ethoxy]sulfonic acid

Details on test material:

- Name of test material (as cited in study report): p-Base-Ester
Batch no.: Op. 1345/1987
CoA: 65 signed 18. January 1987

Sampling and analysis

Analytical monitoring:

no

Details on sampling:

- Concentrations: 125 mg/L (nominal); 101.3 mg/L (measured)
180 mg/L (nominal); 163.7 mg/L (measured)
250 mg/L (nominal); 159.7 mg/L (measured)
- Sampling method: mid of the tank
- Sampling time: 48 h and 96 h
- Sampling volume: ca. 100 mL

Test solutions

Vehicle:

yes

Details on test solutions:

TS was diluted in dilution water by Ultra-Turrax and ultra sonification and distributed evenly in the tank by means of a glass stirrer

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebrabärbling - Brachydanio rerio (Hamilton-Buchanan)
- Strain: Hamilton-Buchanan
- Source: Hoechst AG Eigenzucht der Gewebetoxikologie, Germany
- Date of eclosion: 15. Oct. 1987 - 24. Nov. 1987
- Delivery date:
- Study start: 29. Feb. 1988
- Study end: 11. Mar. 1988
- Length at study initiation (length definition, mean, range and SD): 2.7 to 3.2 cm; mean: 0.0 cm; SD: 0.14
- Weight at study initiation (mean and range, SD):
- Method of breeding:
- Feeding during test: twice per day


ACCLIMATION
- Acclimation period: at least 14 days
- Acclimation conditions:
- Dilution water (ISO/DIS 7346/1)
- 21 to 23°C
- >= 80% Oxygen saturation
- pH 8.3
- 12 hours illumination
- <= 1 g fish/liter
- Type and amount of food: Tetra Min, Tetra Werke, Melle
- Feeding frequency: twice/day
- Health during acclimation (any mortality observed): -

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Post exposure observation period:

NA

Test conditions

Hardness:

no data

Test temperature:

Test Group: 21.9 to 22.2°C
Control Group: 21.6 to 22.2°C

pH:

Test Group: 7.1 to 8.1
Control Group: 7.4 to 8.0

Dissolved oxygen:

Test Group: 7.2 to 9.2 mg/L
Control Group: 7.1 to 9.0 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: Glass, 30x22x24 cm³, 10 liter
- Aeration: oxygen saturated water was used, no aeration during test
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): at least one
- No. of vessels per control (replicates): at least one
- No. of vessels per vehicle control (replicates): NA
- Biomass loading rate: <= 1 g fish/L water


TEST MEDIUM / WATER PARAMETERS
- Intervals of water quality measurement: 2-4, 24, 48, 72, 96 h

OTHER TEST CONDITIONS
- Adjustment of pH: 8.1
- Photoperiod: 12/12 h
- Light intensity: ca. 700 Lux


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : 2-4, 24, 48, 72, 96 h
mortality, appearence, behavior, necropsy

Reference substance (positive control):

not required

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

125 mg/L

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

150 mg/L

Effect concentrations 3

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

180 mg/L

Details on results:

- Behavioural abnormalities: At 180 and 250 mg/l less activity and other swimming position
- Observations on body length and weight: NA
- Other biological observations: At 250 mg/l gill covers standing away
- Mortality of control: no control
- Other adverse effects control: no control
- Abnormal responses: at 125 mg/l enhanced breathing rate and startle response
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: little clouding at 125 mg/l. At 180 and 250 mg/l clouding and precipitation
- Effect concentrations exceeding solubility of substance in test medium: very little
- Other: no effects

Results with reference substance (positive control):

NA

Reported statistics and error estimates:

NA

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

no mortality was observed after 96 hours exposure to 125 mg/L parabaseester
no mortality was observed after 48 hours exposure to 180 or 250 mg/L parabaseester
100% mortality was observed after 96 hours exposure to 180 or 250 mg/L parabaseester