3,7-dimethyloct-6-enenitrile

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non-GLP compliant non-guideline study, adequate for assessment.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1980

Report date:

1980

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

The procedure followed in the present protocol mimic as closely as possible those described by Ames et al. (1975).

GLP compliance:

not specified

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

3,7-dimethyloct-6-enenitrile

EC Number:

257-288-8

EC Name:

3,7-dimethyloct-6-enenitrile

Cas Number:

51566-62-2

Molecular formula:

C10H17N

IUPAC Name:

3,7-dimethyloct-6-enenitrile

Details on test material:

- Name of test material: Citronellylnitril T-04526, No 167849, 16.9.80
No further data

Method

Target gene:

histidine gene

Species / strain

Species / strain / cell type:

S. typhimurium, other: TA 1535, TA 1537, TA 1538, TA98, and TA 100

Metabolic activation:

with and without

Metabolic activation system:

S9 mix of Aroclor-induced rats

Test concentrations with justification for top dose:

0, 0.0007, 0.002, 0.007, 0.02, 0.06 mg test liquid per 0.1 mL methanol per plate.

Vehicle / solvent:

methanol

Controls

Controls 1

Untreated negative controls:

no

Negative solvent / vehicle controls:

yes

True negative controls:

no

Positive controls:

yes

Positive control substance:

sodium azide

Remarks:

Strains: TA 1535, TA 100, without metabolic activation

Controls 2

Untreated negative controls:

no

Negative solvent / vehicle controls:

yes

True negative controls:

no

Positive controls:

yes

Positive control substance:

other: hycanthone methanesulphonate

Remarks:

Strains: TA 1537, TA 1538, TA 98, without metabolic activation

Controls 3

Untreated negative controls:

no

Negative solvent / vehicle controls:

yes

True negative controls:

no

Positive controls:

yes

Positive control substance:

other: 2-aminoanthracene

Remarks:

all strains, with metabolic activation

Details on test system and experimental conditions:

METHOD OF APPLICATION: in agar (plate incorporation)

DURATION: After the top agar has been allowed to harden, the plates are incubated at 37°C for three days.

NUMBER OF REPLICATIONS: 3

DETERMINATION OF CYTOTOXICITY
- Method: inhibition of growth of the backgroun lawn

Evaluation criteria:

Not specifed

Statistics:

No data

Results and discussion

Test results

Species / strain:

S. typhimurium, other: TA 1535, TA 1537, TA 1538, TA 98, TA 100

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Remarks:

Strain TA 1537

Vehicle controls validity:

valid

Untreated negative controls validity:

not examined

Positive controls validity:

valid

Additional information on results:

- The dose range used in the assay was based on a preliminary test performed to assess the toxicity of the compound for the bacteria. 0.1 mg test liquid per plate appeared slightly toxic as revealed by a less dense background lawn of bacterial growth.
- There were no signs that chemical toxicity interfered with the mutagenicity testing: minimal signs of chemical toxicity were only seen with strain TA 1537 in the presence of the S-9 mix at the highest level.

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.