Disodium 5-[[4-[(2-bromo-1-oxoallyl)amino]-2-sulphonatophenyl]azo]-4-hydroxy-6-(methylamino)naphthalene-2-sulphonate

Water solubility

Link to relevant study record(s)

Reference

Endpoint:

water solubility

Type of information:

experimental study

Adequacy of study:

key study

Study period:

22 July 2014 to 25 July 2014

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 105 (Water Solubility)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method A.6 (Water Solubility)

Deviations:

no

GLP compliance:

no

Type of method:

flask method

Water solubility:

7.1 g/L

Temp.:

20 °C

Remarks on result:

other: No data on pH

Conclusions:

The water solubility of the Reactive Red 66 was determined to be 7.1 g/l.

Executive summary:

The water solubility test was conducted with FAT 92354/B according to EU A.6 and OECD 105 guidelines using the flask method. Three flasks were prepared with about 1000 mg test item and 5 ml water. The batches were stirred for different times at 30 °C (experiment A for 24 h, experiment B for 48 h, experiment C for 72 h) in tightly closed flasks. After equilibration (further stirring for 24 h at 20 °C), a saturated solution with a precipitate of the test item could be observed. Aliquots were taken and centrifuged (30 minutes with 5000 rpm at 20 °C). Subsequently, the solution was filtered over 0.20 μm microfilters. The solution is red coloured and analysed by VIS spectroscopy (532 nm). Based on the study results, the water solubility (cs) of FAT 92354/B at a temperature of 20 °C was determined to be 7.1 g/l.

Description of key information

The water solubility of FAT 92354/B at a temperature of 20 °C was determined to be 7.1 g/l.

Key value for chemical safety assessment

Water solubility:

7.1 g/L

at the temperature of:

20 °C

Additional information

The water solubility test was conducted with FAT 92354/B according to EU A.6 and OECD 105 guidelines using the flask method. Three flasks were prepared with about 1000 mg test item and 5 ml water. The batches were stirred for different times at 30 °C (experiment A for 24 h, experiment B for 48 h, experiment C for 72 h) in tightly closed flasks. After equilibration (further stirring for 24 h at 20 °C), a saturated solution with a precipitate of the test item could be observed. Aliquots were taken and centrifuged (30 minutes with 5000 rpm at 20 °C). Subsequently, the solution was filtered over 0.20 μm microfilters. The solution is red coloured and analysed by VIS spectroscopy (532 nm). Based on the study results, the water solubility (cs) of FAT 92354/B at a temperature of 20 °C was determined to be 7.1 g/l.