tetrasodium 2-(4-fluoro-6-(methyl-(2-(sulfatoethylsulfonyl)ethyl)amino)-1,3,5-triazin-2-ylamino)-5-hydroxy-6-(4-methyl-2-sulfonatophenylazo)naphthalene-1,7-disulfonate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From Oct. 12, 1999 to Oct. 14, 1999

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

Analysis of the test concentration and control was carried out at 0 and 48 h.

Vehicle:

no

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Strain: Straus
- Sex: Parthenogenetic females
- Source: Cloned at the Federal Health Office (BGA) in Berlin. Laboratory bred at Bayer AG Leverkusen.
- Age at study initiation: 0 - 24 h

ACCLIMATION
- In M4-Medium (according to Elendt and BGA, 1992)

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Hardness:

258.1 mg CaCO3/L

Test temperature:

Control: 20.1°C
Test: 19.9°C

pH:

Control: 8.1
Test: 8.0

Dissolved oxygen:

Control: 8.5 mg/L
Test: 8.6 mg/L

Nominal and measured concentrations:

- Nominal concentration: 100 mg/L
- Measured concentration:
Arithmetic mean of analytical values (0 h and 48 h): ≥110% of nominal

Details on test conditions:

- Test vessel: Cylindrical test vessels (diameter 4.0 cm, height 6.5 cm)
- Volume: 20 mL test medium
- Ventilation: Not ventilated
- Method of test substance administration: Direct weighing
- Water for dilution: M4-Medium (according to Elendt and BGA, 1992)
- No. of organisms per vessel: 10
- Biomass loading rate: 2 mL per daphnid
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2

OTHER TEST CONDITIONS
- Photoperiod: 16 h light and 8 h dark
- Test concentrations: 100 mg/L (nominal concentration)

Reference substance (positive control):

no

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Table 1: Immobilization of Daphnia

Group	Nominal concentration of test substance (mg/L)	No. of Animals	% immobility
			24 h	48 h
Control	-	20	0	0
Test	100	20	0	0

Validity criteria fulfilled:

yes

Conclusions:

Under the test conditions, the 48 h EC50 and NOEC of the test substance were >100 mg/L and 100 mg/L (nominal concentrations), respectively.

Executive summary:

A limit test was conducted to determine the acute toxicity of the test substance to Daphnia magna according to EU Method C.2, in compliance with GLP.

In this test, 2 replicates of 10 daphnids were exposed to the substance at a nominal concentration of 100 mg/L (mean measured concentration of ≥110 mg/L; estimated by TOC method) under static conditions for 48 h. Immobility was recorded at 24 and 48 h.

 

Under the test conditions, the 48 h EC50 and NOEC of the substance were >100 mg/L and 100 mg/L (nominal concentrations), respectively.

Description of key information

Under the test conditions, the 48 h EC50 and NOEC of the test substance were >100 mg/L and 100 mg/L (nominal concentrations), respectively.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

EC50

Effect concentration:

100 mg/L

Additional information

A limit test was conducted to determine the acute toxicity of the test substance to Daphnia magna according to EU Method C.2, in compliance with GLP. In this test, 2 replicatesof 10 daphnids were exposed to the substance at a nominal concentration of 100 mg/L (mean measured concentration of ≥110 mg/L; estimated by TOC method) under static conditions for 48 h. Immobility was recorded at 24 and 48 h. Under the test conditions, the 48 h EC50 and NOEC of the substance were >100 mg/L and 100 mg/L (nominal concentrations), respectively (Caspers, 1999a).