tetrasodium 2-(4-fluoro-6-(methyl-(2-(sulfatoethylsulfonyl)ethyl)amino)-1,3,5-triazin-2-ylamino)-5-hydroxy-6-(4-methyl-2-sulfonatophenylazo)naphthalene-1,7-disulfonate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From October 25, 1999 to October 29, 1999

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

Daily analysis of the test concentration and control.

Vehicle:

no

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra fish
- Strain: HAMILTON BUCHANAN
- Source: Bio International B.V. (Netherlands)
- Date of birth: 1999-03-04
- Length at study initiation: 2.5 - 3.5 cm

ACCLIMATION
- Acclimation period: 3 months
- Acclimation conditions: Synthetic fresh water in accordance with ISO
- Health during acclimation: Mortality <2%

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

245.6 mg CaCO3/L

Test temperature:

Control: 21.0-21.5°C
Test: 21.0-21.2°C

pH:

Control: 7.6-8.1
Test: 7.7-7.9

Dissolved oxygen:

Control: 8.1-8.8 mg/L
Test: 8.0-9.6 mg/L

Nominal and measured concentrations:

- Nominal concentration: 100 mg/L
- Average measured concentration (0-96 h): 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Ventilated test vessel holding 5 L of test media

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Synthetic fresh water in accordance with ISO

OTHER TEST CONDITIONS
- Photoperiod: 16 h light/8 h dark

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Sublethal observations / clinical signs:

Table 1: Results of acute fish toxicity study

Time [h]	LC 0 [mg/L] (nominal test substance concentration)
2	≥100
24	≥100
48	≥100
72	≥100
96	≥100

Validity criteria fulfilled:

yes

Conclusions:

Under the test conditions, the 96 h LC50 and NOEC of the test substance were found to be >100 mg/L and ≥100 mg/L (nominal concentrations), respectively.

Executive summary:

A study was conducted to determine the acute toxicity of the test substance to Zebra fish (Danio rerio) according to EU Method C.1, in compliance with GLP.

Groups of ten fish were exposed to a concentration of 100 mg/L (nominal) of the test substance dissolved in water. Verification of dose was conducted by TOC analysis at 0, 24, 48, 72 and 96 h. The average measured concentration of the test substance corresponded to 100% of the nominal value. Mortality was recorded at 2, 24, 48, 72 and 96 h. No deaths occurred in the control or test substance treated groups.

Under the study conditions, the 96 h LC50 and NOEC of the test substance were found to be >100 mg/L and 100 mg/L (nominal concentrations), respectively.

Description of key information

Under the test conditions, the 96 h LC50 and NOEC of the test substance were found to be >100 mg/L and 100 mg/L (nominal concentrations), respectively.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

LC50

Remarks:

no mortality observed up to the highest dose level

Effect concentration:

100 mg/L

Additional information

A study was conducted to determine the acute toxicity of the test substance to Zebra fish (Danio rerio) according to EU Method C.1, in compliance with GLP. Groups of ten fish were exposed to a concentration of 100 mg/L (nominal) of the test substance dissolved in water. Verification of dose was conducted by TOC analysis at 0, 24, 48, 72 and 96 h. The average measured concentration of the test substance corresponded to 100% of the nominal value. Mortality was recorded at 2, 24, 48, 72 and 96 h. No deaths occurred in the control or test substance treated groups. Under the study conditions, the 96 h LC50 and NOEC of the test substance were found to be >100 mg/L and 100 mg/L (nominal concentrations), respectively (Müller, 1999).