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Toxicological information

Acute Toxicity: inhalation

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Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Study period:
1963
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study done before GLP was established. Read across justification is given in section 13 assessment reports: WF6 justification for read across

Data source

Reference
Reference Type:
publication
Title:
A toxicopathologic study in animals after brief single exposures to hydrogen fluoride
Author:
Rosenholtz MJ, Carson TR, Weeks MH, Wilinski F, Ford DF & Oberst FW; Rosenholtz MJ, Carson TR, Weeks MH, Wilinski F, Ford DF & Oberst FW;
Year:
1963
Bibliographic source:
publication Am. Ind. Hyg. Assoc. J. 24:253-261, 1963

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Acute inhalation toxicity in the rat.
Maximum exposure: 60 min.
GLP compliance:
no
Remarks:
done before GLP was established
Test type:
standard acute method
Limit test:
no

Test material

Constituent 1
Reference substance name:
Hydrogen fluoride
EC Number:
231-634-8
EC Name:
Hydrogen fluoride
Cas Number:
7664-39-3
IUPAC Name:
fluoride
Test material form:
other: HF vapour

Test animals

Species:
rat
Strain:
Wistar
Sex:
male
Details on test animals or test system and environmental conditions:
Weight: 100 - 120 g

Administration / exposure

Route of administration:
inhalation: vapour
Type of inhalation exposure:
whole body
Vehicle:
other: no vehicle
Details on inhalation exposure:
Volume of the exposure chamber/housing was 400 l with an air flow of 200 l/min. An expansion tank and a manometer were used.
Constant temperature at 20 degree Celsius.
Analytical verification of test atmosphere concentrations:
yes
Duration of exposure:
60 min
Remarks on duration:
5, 15, 30 and 60 min
Concentrations:
various
No. of animals per sex per dose:
20 male rats per group/concentration
Control animals:
yes
Details on study design:
Groups of rats were exposed to various concentrations of hydrogen fluoride vapour for either 5, 15, 30 or 60 minutes. Surviving rats were weighed daily and observed for 14 days after exposure. Clinical observations were made following exposure, body weights were recorded daily and mortalities recorded.
Statistics:
The LC50 values for each exposure time were calculated using the method of Bliss (1952).

Results and discussion

Effect levelsopen allclose all
Sex:
male
Dose descriptor:
LC50
Effect level:
4 970 ppm
Based on:
test mat.
95% CL:
4 584 - 5 388
Exp. duration:
5 min
Remarks on result:
other: slope: 11.8 std. dev. +/- 4.2
Sex:
male
Dose descriptor:
LC50
Effect level:
2 689 ppm
Based on:
test mat.
95% CL:
2 397 - 3 016
Exp. duration:
15 min
Remarks on result:
other: slope: 9.4 std. dev. +/- 2.1
Sex:
male
Dose descriptor:
LC50
Effect level:
2 042 ppm
Based on:
test mat.
95% CL:
1 901 - 2 192
Exp. duration:
30 min
Remarks on result:
other: slope: 14.0 std. dev. +/- 3.3
Sex:
male
Dose descriptor:
LC50
Effect level:
1 307 ppm
Based on:
test mat.
95% CL:
1 212 - 1 410
Exp. duration:
60 min
Remarks on result:
other: slope: 10.7 std. dev. +/- 3.8
Mortality:
Mortality occurred, but count of rats that passed away was not reported. Some deaths were reported to occur during the post-exposure observation period.
Clinical signs:
other: Conjunctival and nasal irritation as shown by reddened conjunctivae, pawing at the nose, marked lacrimation, nasal secretion and sneezing. These signs were not seen 1 week following exposure (in surviving animals). Respiratory distress and general weaknes
Body weight:
Body weight loss was observed in surviving animals. Compared to controls, surviving rats exhibited a 10-15% reduction in body weight during the first 3-7 days after exposure, after which weights returned to normal.
Gross pathology:
Lesions were found (differing quantitatively from that of controls) in the kidneys, liver, nasal passage, bone marrow and skin of exposed rats.

Any other information on results incl. tables

The LC50 values were 4970, 2690, 2040 and 1310 ppm for rats exposed to hydrogen fluoride vapour for 5, 15, 30 and 60 minutes, respectively.

Applicant's summary and conclusion

Interpretation of results:
very toxic
Remarks:
Migrated information Criteria used for interpretation of results: EU
Conclusions:
The LC50 values were 4970, 2690, 2040 and 1310 ppm for rats exposed to hydrogen fluoride vapour for 5, 15, 30 and 60 minutes, respectively.
1310 ppm HF equals 218 ppm WF6 and 2700 mg/m³ of WF6 at 20 °C and 1,013 bar
Executive summary:

Male Wistar rats were exposed to lethal concentrations of hydrogen fluoride vapour, at various concentrations for either 5, 15, 30 or 60 minutes. The LC50 values were 4970, 2690, 2040 and 1310 ppm for rats exposed to hydrogen fluoride vapour for 5, 15, 30 and 60 minutes, respectively. Exposure resulted in mortalities, although numbers and times of death are not reported. Lesions were observed in the kidneys, liver, nasal passages, bone marrow and skin. Exposure resulted in conjunctival and nasal irritation, respiratory distress and general weakness and body weight loss.