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Ecotoxicological information

Toxicity to microorganisms

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Description of key information

DIN 38 412 part 8 (Pseudomonas putida), : EC10(16h) >= 8 g/L, EC50(16h) >= 8 g/L (based on growth inhibition) 

Key value for chemical safety assessment

EC50 for microorganisms:
8 000 mg/L
EC10 or NOEC for microorganisms:
8 000 mg/L

Additional information

The toxic effect on growth of Pseudomonas putida of '1,3,4-Thiadiazolidine-2,5-dithione, reaction products with hydrogen peroxide and tert-dodecanethiol' (no CAS#, no EC#) was investigated according to German standard DIN 38 412. The test temperature was 21 °C and the test duration 16 h. Test concentrations were used as 80 mg/L and 8000 mg/L. Cultivation of the pre-culture was done 7 h before inoculation at 21 °C. An aqueous extract was prepared according to DEV S 4 using 100 mg test substance. This amount was mixed with 1 L deionised water and shaked overhead for 24 h. Filtration was done with the help of a rough fluted filter and the filtrate was used for testing. In the second preparation, an eluate with 10 g/L test substance was used. The test was conducted in Erlenmeyer flasks on a shaker with incubation hood. Up to the maximal water solubility, the test substance was not toxic to Pseudomonas putida and possesses no toxic, water soluble ingredient or impurity. Therefore, the EC10(16h) was determined to be >= 8 g/L (based on growth inhibition); the same applies for the EC50(16h) value.