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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- September - November 2012
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- yes
- Remarks:
- Because of the poor solubility of the test item, no calculation from content of organic carbon of the test item and DOC measurement was performed.
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
The water-accommodated fractions were prepared for the test. This was done mixing the nominal loads with the corresponding amount of nutrient medium and shaking vigorously for 24 hours. The resulting solutions were filtrated through 0.45 µm filters. - Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: green alga (Desmodesmus subspicatus)
- Strain: CHODAT
- Source, Method of cultivation: The culture of Desmodesmus subspicatus was obtained in Oct. 2011 by MBM Sciencebridge GmbH (Institut für Pflanzenphysiologie of Universität Göttingen). The algae are kept as stock culture on solid agar at 8 °C. From the stock culture, a permanent culture was prepared. From an aliquot of the permanent culture, the pre-culture was prepared.
- Age of inoculum (at test initiation):
Four days before the start of each test, an aliquot of the stock culture containing a few cells was brought into pre-culture medium and incubated for 96 hours. The resulting culture is growing exponentially. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Post exposure observation period:
- Not applicable
- Hardness:
- Not reported.
- Test temperature:
- 24 °C
- pH:
- 7.7 - 8.6
- Dissolved oxygen:
- Not applicable
- Salinity:
- Not applicable
- Nominal and measured concentrations:
- 4.6 / 10 / 22 / 46 / 100 mg/L nominal concentration
- Details on test conditions:
- TEST SYSTEM
- Test vessel: glass flasks, total volume 50 mL
- Aeration: No
- Initial cells density: 2 *10^3 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes
OTHER TEST CONDITIONS
- Sterile test conditions: Yes
- Adjustment of pH: No
- Photoperiod: Not reported
- Light intensity and quality: 5800 Lux
- Salinity (for marine algae): n.a.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: calculated based on the determination of the absorption at 440 nm
- At the end of the test, the treatments were examined microscopically in order to assess the appearance of the alga and detect abnormalities (e.g. caused by the exposure to the test item). - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate K2Cr2O7 (CAS No. 7778-50-9)
- Duration:
- 72 h
- Dose descriptor:
- NOELR
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EL10
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EL50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOELR
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Duration:
- 72 h
- Dose descriptor:
- NOELR
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Details on results:
- - Exponential growth in the control (for algal test): Yes
- Observation of abnormalities (for algal test):
- Unusual cell shape: No
- Colour differences: No
- Flocculation: No
- Adherence to test vessels: No
- Aggregation of algal cells: No
- Other: None
- Any stimulation of growth found in any treatment: No
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: No
- Effect concentrations exceeding solubility of substance in test medium: No - Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- ErC50: 1.1 mg/L
- Other: NOErC = 0.16 mg/L - Reported statistics and error estimates:
- t-test
F-test - Validity criteria fulfilled:
- yes
- Conclusions:
- In a GLP-study according to OECD Test Guideline 201, the registered substance was not toxic to algae up to a nominal concentration of 100 mg/L.
- Executive summary:
A study according to OECD 201 (resp. EU C.3) was performed to evaluate the toxicity of the registered substance to the alga Desmodesmus subspicatus.
The study was performed using five concentrations ranging from 4.6 to 100 mg/L nominal concentration. Incubation time (test system Desmodesmus subspicatus) was 72 hours. The cell concentration of each replicate was determined by measuring the absorption of the solutions at 440 nm every 24 hours with a spectral photometer. The cell density of the cultures was calculated based on the correlation curve between the adsorption and the cell density of the cultures determined by microscope counts. Growth rate µ, area under the growth curve (AUC) and the yield were determined from the cell densities at the respective observation times.
Significant inhibition of algal growth was not observed in any of the treatments compared to the control.
At the beginning and at the end of the test, the content of the test item in the test solutions was determined using DOC-determination. Because of the very low solubility in test medium, no calculation from content of carbon of the test item and DOC measurement was possible. The measured start concentration in the treatments was only slightly above the LOQ. At the end of the test, the concentration of DOC in the treatments and in the control was in the same range. That means that in presence of the test system, the DOC was affected by the algae.
Therefore, the determination of the biological results was based on the nominal loadings.
The EC50s of potassium dichromate were tested in a separate reference test (GLP study no. 201206R301). The values lay within the normal range of the laboratory.
The following results for the test item CARDOLITE NC-513 were determined:
Endpoint
NOEL
LOEL
EL10
EL50
Growth Rate
≥ 100 mg/L
> 100 mg/L
> 100 mg/L
> 100 mg/L
AUC 1
≥ 100 mg/L
> 100 mg/L
> 100 mg/L
> 100 mg/L
Yield 2
≥ 100 mg/L
> 100 mg/L
> 100 mg/L
> 100 mg/L
1 AUC (Area Under Curve according to EU Method C.3) means the integral of the biomass. Calculation see under 8.3.
2 Yield (according to OECD Guideline 201) is defined as the biomass at the end of the exposure period minus the biomass at the start of the exposure period. Calculation see under 8.4
Reference
Growth Rate µ, Area under the Curve AUC, Yield Main Study
NominalConcentrationin mg/L |
Growth Rate [day-1] |
AUC |
Yield |
|
0 |
Mean |
1.43 |
138978 |
159200 |
SD |
0.05 |
15556 |
20819 |
|
4.6 |
Mean |
1.39 |
119859 |
143390 |
SD |
0.06 |
18244 |
25797 |
|
10 |
Mean |
1.35 |
114161 |
125374 |
SD |
0.05 |
17123 |
19895 |
|
22 |
Mean |
1.48 |
158097 |
182363 |
SD |
0.01 |
4176 |
7091 |
|
46 |
Mean |
1.39 |
125374 |
141919 |
SD |
0.09 |
28242 |
36488 |
|
100 |
Mean |
1.56 |
238616 |
240454 |
SD |
0.05 |
41094 |
36164 |
SD = Standard Deviation
Description of key information
- EL50 (72h) for freshwater algae (Pseudokirchneriella subcapitata): >100 mg/l [nominal; based on both growth rate and yield; OECD 201]
- NOEL (72h) for freshwater algae (Pseudokirchneriella subcapitata): ≥100 mg/l [nominal; based on both growth rate and yield; OECD 201]
- LOEL (72h) for freshwater algae (Pseudokirchneriella subcapitata): >100 mg/l [nominal; based on both growth rate and yield; OECD 201]
Key value for chemical safety assessment
- EC10 or NOEC for freshwater algae:
- 100 mg/L
Additional information
In a GLP-study according to OECD Test Guideline 201, the registered substance was not toxic to algae up to a nominal concentration of 100 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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