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EC number: 271-178-7 | CAS number: 68516-75-6
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- Aquatic toxicity
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- Short-term toxicity to fish
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Toxicity to soil macroorganisms except arthropods
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to soil macroorganisms except arthropods: short-term
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 2012-02-02 to 2012-02-17
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
- Version / remarks:
- and ISO 11268-1 (1997)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on preparation and application of test substrate:
- - Method of mixing into soil (if used):
The limit test item concentration was weighed out and dissolved in acetone (2.5 mL per replicate). The dissolved test item was mixed thoroughly with quartz sand (10 g per replicate). After complete evaporation of the solvent, the treated quartz sand was given to the artificial soil. Additional demineralised water was added to the artificial soil to adjust the humidity of the artificial soil to a moisture of 40 - 60% of the maximum water holding capacity.
Subsequently, the test medium was thoroughly mixed to ensure a homogeneous distribution and about 555 g soil dry weight were filled into each test vessel.
- Controls: Artificial soil moistened with demineralised water without test item was used as control medium.
- Solvent Control: Artificial soil with addition of quartz sand treated with acetone and moistened with demineralised water without test item was used as solvent control medium. - Test organisms (species):
- Eisenia fetida
- Animal group:
- annelids
- Details on test organisms:
- TEST ORGANISM
- Common name: Earthworm Eisenia fetida Savigny 1826
- Source: Own breeding
- Age at test initiation: Adult
- Weight at test initiation (mean and range, SD): individual live weight of 0.30 to 0.60 g
Control: 0.48 +/- 0.03, Solvent Control: 0.48 +/- 0.03; 1000 mg/kg: 0.48 +/- 0.04
ACCLIMATION
- Acclimation period: 2 days
- Acclimation conditions (same as test or not): same as test
- Health during acclimation (any mortality observed): No mortality observed - Study type:
- laboratory study
- Substrate type:
- artificial soil
- Limit test:
- yes
- Total exposure duration:
- 14 d
- Post exposure observation period:
- No
- Test temperature:
- nominal: 20 +/- 2 °C, actual: 18 - 21 °C
- pH:
- Test item concentration
[mg/kg soil dry weight]]
pH-value
day 0 day 14
Control 5.13 5.03
Solvent Control 5.10 4.99
1000 5.08 5.01 - Moisture:
- Test item concentration
[mg/kg soil dry weight]] Moisture [%] of dry weight
day 0 day 14
Control 33.9 33.3
Solvent Control 33.0 32.8
1000 32.9 33.6 - Details on test conditions:
- TEST SYSTEM
- Test container (material, size): Glass dishes, covered with perforated plastic film
- Amount of soil or substrate: 555 g (dry weight)
- No. of organisms per container (treatment): 10 per replicate
- No. of replicates per treatment group: 4
- No. of replicates per control, solvent control: 4
SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Composition (if artificial substrate): 10 % peat (air dried and finely ground), 20 % kaolin (kaolinite content 84 - 87 %), 69 % quartzsand (sand with > 50 % particles size of 0.05 to 0.2 mm), 0.39% calcium carbonate (CaCO3) to adjust the pH to 6.0 ± 0.5
- Organic carbon (%): 10 % peat
- Maximum water holding capacity (in % dry weigth): 69
- Storage (condition, duration): under test conditions for 9 days
- Pretreatment of soil: Two days before earthworm insertion into the test vessels the artificial soil (10 kg) was pre-moistened with 1100 g deionised water to achieve a soil moisture of 26 % of the maximum water holding capacity of the artificial soil. Until start of the test evaporation of the artificial soil was avoided.
OTHER TEST CONDITIONS
- Photoperiod: 24 h
- Light intensity: 400 - 800 lx
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
The live weight of the earthworms was measured individually at the day of application (day 0) and at day 14.
Mortality, behaviour and morphological changes of the earthworms were recorded 7 and 14 days after application.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: none, limit test
- Range finding study
Range Finding Test (14 d, non - GLP): Mortality and Change of Live Weight [%]
Concentration
Test item
Mortality Change of Live weight
[mg/kg Soil dry weight] [%] [%]
Control 0 -10
Solvent Control 0 -8
10 0 -12
100 0 -11
1000 0 -7 - Nominal and measured concentrations:
- Nominal: 1000 mg/kg soil DW
- Reference substance (positive control):
- yes
- Remarks:
- 2-Chloroacetamide
- Duration:
- 14 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks:
- loss of body weight, behaviour and pathological symptoms
- Details on results:
- - Mortality at end of exposure period: No evident mortality (<10 %) occurred in the controls as well as at the limit test item concentration after 14 days of exposure to the test item
- Changes in body weigth of live adults (% of initial weight) at end of exposure period:
During the test the biomass loss of the earthworms was below 20 % in the control. There was no statistically or biologically significant difference between earthworm biomass development in the pooled control and in the limit test item group
- Morphological abnormalities: No pathological symptoms or changes in the earthworm behaviour were observed in the control as well as in the limit test item concentration after 7 and 14 days of exposure
- Other biological observations: None - Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- The LC50-value ranged between 20 and 80 mg/kg soil dry weight as recommended by the guideline
please refer to "Any other information on materials and methods" - Reported statistics and error estimates:
- Because no biological differences were observed, both controls were pooled for statistical evaluation.
A t-test was carried out for the determination of statistically significant differences compared to the pooled control - Validity criteria fulfilled:
- yes
- Conclusions:
- No evident mortality (<10%) as well as no pathological symptoms or changes in the earthworm behaviour were observed in the control and solvent control as well as at the limit test item concentration of 1000 mg test substance /kg soil dry weight. Compared to the pooled control, no statistically or biologically significant difference of biomass was observed in the limit test item concentration at test end.
Consequently, the No Observed Effect Concentration (NOEC) with respect to mortality, loss of body weight and other symptoms was determined to be at 1000 mg test substance /kg soil dry weight.
Reference
Earthworm Mortality in [%] after 7 and 14 Days of Exposure
Mortality [%] |
||||||||||
Test item concentration [mg/kg soil dry weight] |
Day 7 |
Day 14 |
||||||||
1 |
2 |
3 |
4 |
MV |
1 |
2 |
3 |
4 |
MV |
|
Control |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
Solvent Control |
0 |
0 |
0 |
10 |
2.5 |
0 |
0 |
0 |
10 |
2.5 |
1000 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
Earthworm Behaviour and Pathological Symptoms after 7 and 14 Days of Exposure
Test item concentration [mg/kg soil dry weight] |
Effect |
Day 7 Replicate |
Day 14 Replicate |
||||||
1 |
2 |
3 |
4 |
1 |
2 |
3 |
4 |
||
Control |
A) |
10/10 |
10/10 |
10/10 |
10/10 |
10/10 |
10/10 |
10/10 |
10/10 |
Solvent Control |
A) H) |
10/10 |
10/10 |
10/10 |
9/10 1/10 |
10/10 |
10/10 |
10/10 |
9/10 1/10 |
1000 |
A) |
10/10 |
10/10 |
10/10 |
10/10 |
10/10 |
10/10 |
10/10 |
10/10 |
Mean Biomass and Mean Loss of Body Weight
Mean live weight |
|||||
Test item concentration [mg/kg soil dry weight] |
Test start MV ± SD [g/worm] |
Test end MV ± SD [g/worm] |
Test end CV [%] |
Change of live weight [%] |
Statistically significant vs. Pooled Control |
Control |
0.48 ± 0.03 |
0.48 ± 0.04 |
8 |
0 |
– |
Solvent Control |
0.48 ± 0.03 |
0.46 ± 0.05 |
11 |
- 4 |
|
1000 |
0.48 ± 0.04 |
0.48 ± 0.04 |
8 |
0 |
No |
Description of key information
In a toxicity study with Esenia fetida (OECD 207), no toxic effects up to 1000 mg test substance /kg soil dry weight was observed. As such, no hazard was concluded.
Key value for chemical safety assessment
Additional information
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