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EC number: - | CAS number: -
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
Description of key information
Not readily biodegradable; OECD 301B, EU Method C.4-C, ISO 9439 and ISO 10634
Key value for chemical safety assessment
- Biodegradation in water:
- inherently biodegradable
Additional information
The potential of the test material to undergo biodegradation was investigated in a modified Sturm test conducted in accordance with the standardised guidelines OECD 301B, EU Method C.4-C, ISO 9439 and ISO 10634 under GLP conditions.
The study was awarded a reliability score of 1 in accordance with the principles for assessing data quality set forth by Klimisch et al. (1997).
Activated sludge from a municipal sewage treatment plant receiving predominantly domestic sewage was exposed to the test material for 75 days, with the last CO2 measurement on day 76. The test material was tested in duplicate at approximately 17 mg/L, corresponding to 12 mg TOC/L. Based on the TOC content, the ThCO2 of the test material was calculated to be 2.64 mg CO2/mg.
Since the test material was not sufficiently soluble to allow preparation of an aqueous solution at a concentration of 1 g/L, weighed amounts were added to 2-litre test bottles containing medium with microbial organisms and mineral components. To this end, 10 mL of Milli-RO water was added to each weighing bottle containing the test material. After vigorous vortex mixing, the resulting slightly foaming suspension was added quantitatively to 300 mL Milli-RO and this mixture was vigorously stirred for 15 minutes. Thereafter, the inoculated medium was added and made-up to 2 L with Milli-RO water. The test solutions were continuously stirred during the test, to ensure optimal contact between the test material and the test organisms.
The relative biodegradation values calculated from the measurements performed during the test period revealed 17 and 21 % biodegradation of the test material for A and B, respectively, on day 29 and 59 and 42 % biodegradation at the end of the prolonged test period (75 days, last titration day 76). Thus, biodegradation of at least 60 % was not reached within the test period.
In the toxicity control, more than 25 % biodegradation occurred within 14 days (28 %, based on ThCO2). Therefore, the test material was assumed not to inhibit microbial activity. All criteria for acceptability of the test were met, therefore the study was considered to be valid.
Under the conditions of this study, the test material was determined not to be readily biodegradable.
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