Pregna-1,4,6-triene-3,20-dione, 17-hydroxy-

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Nov 1995

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1995

Report date:

1996

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Version / remarks:

1992

Test guideline 2

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

adopted: 04 Apr. 1984

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

17-Hydroxy-1,4,6-pregnatriene-3,20-dione

EC Number:

613-906-1

Cas Number:

66212-25-7

Molecular formula:

C21 H26 O3

IUPAC Name:

17-Hydroxy-1,4,6-pregnatriene-3,20-dione

Test material form:

solid: bulk

Sampling and analysis

Analytical monitoring:

yes

Remarks:

TOC analysis

Details on sampling:

- Concentrations: saturated solution
- Sampling method: samples taken at 0 and 24 h

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 100.3 mg were suspended in 1000 mL tap water under constant stirring for 24 hours. Thereafter, the solution was filtered through a glass-fibre filter (pore size 0.45 µm). The filtered solution was used as the test solution.
- Eluate: no
- Differential loading: yes
- Controls: yes, test medium control

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Age at study initiation (mean and range, SD): <24 h

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Test temperature:

start: 20.5 - 21.2 °C
end: 20.1 - 20.3 °C

pH:

start: 7.6 - 7.9
end: 8.6 - 8.7

Dissolved oxygen:

start: 8.0 - 9.1 mg/L
end: 8.5 - 8.6 mg/L

Nominal and measured concentrations:

nominal: saturated solution
measured: 4 mg/L (TOC)

Details on test conditions:

TEST SYSTEM
- Test vessel: 100 mL test vessels
- Volume of solution: 50 mL
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to guideline

OTHER TEST CONDITIONS
- Light intensity: 12 h light/12 h darkness

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Mobility after 24 and 48 h

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 4 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mobility

Any other information on results incl. tables

Table 1: Cumulative immobilization

Test compound	No. of Daphnia	No. of immobilized Daphnia
		24 hours	48 hours
Control	20	0	0
Saturated solution of ZK 5668	20	0	0

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

A saturated solution of 1,4,6 Trienol with the concentration of 4.0 mg/L had no adverse effects on the mobility of Daphnia. Therefore, the EC50/48 hours to Daphnia magna is higher than a saturated solution.

Executive summary:

The purpose of this study was to determine the acute toxicity (immobilization, EC50/48 hours)of 1,4,6 -Trienol to Daphnia magna in a limit-test. The study was conducted in agreement with the test guideline of the OECD, no. 202.

Twenty Daphnia were used tor the test solution of test substance and twenty tor the control group. The Daphnia were exposed to the test substance and the dilution water for a period of 48 hours under static conditions in a limit-test. Immobilization was recorded at 24 hours and 48 hours. In order to establish a saturated solution of this low soluble compound, a suspension of 1,4,6 -Trienol of 100 mg/L was prepared and stirred for 24 hours. Then it was filtered through a glass-fibre filter. This saturated solution obtained was used for the test. Sampies tor a TOC analysis were taken at 0 and 48 hours. The analytical results gave a concentration of 4.0 mg/L based on a calculation using the molecular formula of the test substance. No immobilized Daphnia was observed.