3,7,11-trimethyldodeca-1,6,10-trien-3-ol,mixed isomers

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1994

Report date:

1994

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

3,7,11-trimethyldodeca-1,6,10-trien-3-ol,mixed isomers

EC Number:

230-597-5

EC Name:

3,7,11-trimethyldodeca-1,6,10-trien-3-ol,mixed isomers

Cas Number:

7212-44-4

Molecular formula:

C15H26O

IUPAC Name:

3,7,11-trimethyldodeca-1,6,10-trien-3-ol

Constituent 2

Reference substance name:

Nerolidol

EC Number:

205-540-2

EC Name:

Nerolidol

Cas Number:

142-50-7

Molecular formula:

C15H26O

IUPAC Name:

3,7,11-trimethyldodeca-1,6,10-trien-3-ol

Details on test material:

- Name of test material (as cited in study report):
- Analytical purity: 97.9 %
- Lot/batch No.: 217938

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution was prepared by adding 50.6 to 1000 ml water. The test solutions were prepared separately.

Test organisms

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

TEST ORGANISM
- Source (laboratory, culture collection): Collection of algae cultures Pflanzenphysiologisches Institut University of Goettingen, Germany

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Test conditions

Test temperature:

24 +/- 1 °C

pH:

after 0 h: 7.7 -7.9
after 72 h: 8.0 - 8.2

Nominal and measured concentrations:

Nominal test concentrations (mg/l):
0, 0.44, 0.96, 2.1, 4.6, 10 and 22

Measured test concentrations (initial):
<0.08, 0.39, 0.92, 2.0, 3.9, 9.3, 21.3 mg/l
Measured test concentrations (at 72 h):
<0.08,<0.08,<0.08, 0.27, 2.1, 6.1, 13.0 mg/l

Recovery rate. 97 %

Details on test conditions:

TEST SYSTEM
- Test vessel: Erlenmeyer flasks (100 ml)
- Type (delete if not applicable): closed with cotton stoppers
- Fill volume: 50 ml
- Initial cells density: 9300 cells/ml
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6


OTHER TEST CONDITIONS
- Photoperiod: continous
- Light intensity and quality: cold white fluorescent light, 113 µE/m² sec



EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: Cell numbers were counted (using a TOA cell couner) after 24, 48 and 72 h.
- Chlorophyll measurement:
- Other:

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

0.44 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Reported statistics and error estimates:

McCullagh and Nelder (1983) Generalized linear models. Chapman & Hall. London.