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Diss Factsheets

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
GLP compliance:
yes (incl. QA statement)
Analytical monitoring:
yes
Test organisms (species):
Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
Details on test organisms:
-Test species and strain: Pseudokirchneriella subcapitata KORSHIKOV (SAG 61.81)
- Age (on study day 0): A stock algal culture is maintained continuously at the test facility. Before the exposure an inoculum culture is prepared from the stock culture and incubated for 4 days at 21 – 24 °C (max. temperature difference 2 °C). After this time, the inoculum culture is in exponential growth phase and can be used to initiate the test (study day 0).
- Supplier: Collection of algal cultures in University of Göttingen/Germany
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: Erlenmeyer flasks (nominal volume 250 mL) plugged with gas permeable silicone sponge caps, 100 mL fill volume
- Initial cells density: 0.5 x 104 cells/mL
- No. of vessels per concentration (replicates): 6
- No. of vessels per control (replicates): 6


GROWTH MEDIUM
- USEPA media

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: permanent illumination
- Light intensity and quality: Average 7065 lux (within ±15% variability) at a wave length of 400 - 700 nm
Reference substance (positive control):
yes
Remarks:
Potassium dichromate
Key result
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 293 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: At the nominal concentration of the test substance (293 mg/L) the concentration of the main component is 118 mg/L.
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
>= 293 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: At the nominal concentration of the test substance (293 mg/L) the concentration of the main component is 118 mg/L.
Validity criteria fulfilled:
yes
Conclusions:
In an Algae Growth Inhibition Test according to OECD guideline 201, the EC50 and NOAC were both above 293 mg/L.

Description of key information

EC50 (72 h) > 293 mg/L (nominal, P. subcapitata, OECD 201)


 


At the nominal concentration of the test substance (293 mg/L) the concentration of the main component is 118 mg/L.

Key value for chemical safety assessment

EC50 for freshwater algae:
293 mg/L

Additional information