Disodium succinate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Experimental result performed using standard methods of analysis

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Principles of method if other than guideline:

Acute Immobilization Study of test material on Daphnia magna in a Semi-Static System (48 Hours)

GLP compliance:

no

Analytical monitoring:

not specified

Vehicle:

no

Details on test solutions:

The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 100 mg of the test substance in 100 ml of ADaM’s media. Achieving test concentrations of 1 g/L, respectively

Test organisms (species):

Daphnia magna

Details on test organisms:

- Common name: Daphnia magna
- Source: Own breeding of daphnia magna
-Food: No feeding after hatching
-Photoperiod: 16 hours light and 8 hours dark

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Post exposure observation period:

Observations (immobility, pH, Temperature, dissolved oxygen content) were recorded after 24 and 48hours of the start of the experiment.

Hardness:

150.5 mg of CaCO3

Test temperature:

20±2

pH:

7.1

Nominal and measured concentrations:

100 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: glass beaker
- Aeration: No aeration during experiment
- fill volume: 20ml
- No. of organisms per vessel: 10 daphnids

OTHER TEST CONDITIONS
- Photoperiod:16 hours light and 8 hours dark

Reference substance (positive control):

not specified

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

9.1. Immobility

Nominal Concentration (mg/l)	Number of Daphnids	Immobility
		0 h	24h	48h
Control	10	-	-	-
100	10	-	-	-

 

pH and oxygen content:

Nominal Concentration (mg/l)	Number of Daphnids	pH(mg/L)
		0 h	48h
Control	10	7.5	7.7
100	10	7.6	7.8

 

 

Nominal Concentration (mg/l)	Number of Daphnids	DO(mg/L)
		0 h	48h
Control	10	7.0	6.8
100	10	7.0	6.8

 

Test temperature:

Nominal Concentration (mg/l)	Number of Daphnids	Temparature
		0 h	48h
Control	10	21.2	21.5
100	10	21.2	21.5

 

Validity criteria fulfilled:

yes

Conclusions:

Based on nominal concentrations, experimental median effective Concentrations [EC-50 (48 h)] for test material on test daphnia was found to be > 100 mg/L.

Executive summary:

Daphnia sp., Acute Immobilization Test according to OECD Guideline 203 was conducted for test material. The nominal concentration selected for the experiment were 100mg/L and test Daphnia magna were exposed to these concentration for 48 hours. The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 100 mg of the test substance in 100 ml of ADaM’s media. Achieving test concentrations of 1 g/L, respectively. The median lethal concentration (EC50) for disodium butanedioate on Daphnia magna in a 48 hours study on the basis of immobilization effect was found to be >100 mg/L. Thus, on the basis of this EC50 value and according to CLP criteria for aquatic classification of the substance, it is concluded that the substance, test material does not exhibit short term toxicity to Daphnia. EC50 (48 hours) Experimental = >100 mg/L

Description of key information

Short term toxicity to aquatic invertebrate:

Daphnia magna Acute Immobilization Test according to OECD Guideline 203 was conducted for test material. The nominal concentration selected for the experiment were 100mg/L and test Daphnia magna were exposed to these concentration for 48 hours. The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 100 mg of the test substance in 100 ml of ADaM’s media. Achieving test concentrations of 1 g/L, respectively. The median lethal concentration (EC50) for disodium butanedioate on Daphnia magna in a 48 hours study on the basis of immobilization effect was found to be >100 mg/L. Thus, on the basis of this EC50 value and according to CLP criteria for aquatic classification of the substance, it is concluded that the substance, test material does not exhibit short term toxicity to Daphnia. EC50 (48 hours) Experimental = >100 mg/L

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 mg/L

Additional information

Short term toxicity to aquatic invertebrate:

Toxicity of test material was evaluated for aquatic invertebrate based on study report of the test material and data from authoritative database.

Daphnia sp., Acute Immobilization Test according to OECD Guideline 203 was conducted for test material. The nominal concentration selected for the experiment were 100mg/L and test Daphnia magna were exposed to these concentration for 48 hours. The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 100 mg of the test substance in 100 ml of ADaM’s media. Achieving test concentrations of 1 g/L, respectively. The median lethal concentration (EC50) for disodium butanedioate on Daphnia magna in a 48 hours study on the basis of immobilization effect was found to be >100 mg/L. Thus, on the basis of this EC50 value and according to CLP criteria for aquatic classification of the substance, it is concluded that the substance, test material does not exhibit short term toxicity to Daphnia. EC50 (48 hours) Experimental = >100 mg/L.

The above study report was further supported by data fromauthoritative database.The EC50 value of the test substance was evalauted according to OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) .Test material to Daphnia magna is found to be >1000 mg/L. This value indicates that the substance is not likely to exhibit short term toxicity to aquatic invertebrate.

Based on the above data it can be considered that the test material is not toxic to aquatic invertebrate and can not be classified as per CLP criteria.