Anthracene

Bioaccumulation: aquatic / sediment

Administrative data

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1977

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study

Data source

Reference

Reference 1

Reference Type:

other: data bank

Title:

Anthracene - Biodegradation and Bioconcentration of Existing Chemical Substances under teh Chemical Substances control Law

Author:

NITE

Year:

2002

Bibliographic source:

www.safe.nite.go.jp/data/hazkizon/pk_e_kizon_input_second.home_object

Report date:

1977

Reference 2

Reference Type:

other: data bank

Title:

Unnamed

Report date:

1992

Reference 3

Reference Type:

secondary source

Title:

REQUEST for information on BCF testing (Inquiry into Japanese Studies of Bioconcentration in Fish)

Author:

de Bruijn J

Year:

2003

Bibliographic source:

E.mail message to EU Member States and CEFIC

Report date:

2003

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 305 (Bioconcentration: Flow-through Fish Test)

Version / remarks:

May 12, 1981

Deviations:

yes

Remarks:

Elimination (depuration) not determined

Test guideline 2

Qualifier:

according to guideline

Guideline:

other: Japan Ministry of Health and Welfare as well as MITI No. 1 , 1974

Principles of method if other than guideline:

in accordance with Japanese Industrial Standard (JIS K 0102-1986-71), titled "Testing methods for industrial waste water"

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Anthracene

EC Number:

204-371-1

EC Name:

Anthracene

Cas Number:

120-12-7

Molecular formula:

C14H10

IUPAC Name:

anthracene

Details on test material:

- Name of test material (as cited in study report): anthracene
- Molecular formula (if other than submission substance): C14H10
- Molecular weight (if other than submission substance):
- Substance type: organic
- Physical state: solid
- Analytical purity: >= 96%
- Impurities (identity and concentrations): no data

Radiolabelling:

no

Test solutions

Vehicle:

yes

Details on preparation of test solutions, spiked fish food or sediment:

PREPARATION OF SPIKED WATER
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): see below
- Concentration of vehicle in final test solution:

Test substance[µg/L] Dispersant[µg/L]
_______________________________________________
Tween-80 DMSO
_______________________
15 75 4130
1.5 7.5 413
_______________________________________________

Test organisms

Test organisms (species):

Cyprinus carpio

Details on test organisms:

TEST ORGANISM
- Common name: carp
- Source: Sugishima fish farm, Kumamoto
- Length at study initiation (lenght definition, mean, range and SD): mean 10.6 cm
- Weight at study initiation (mean and range, SD): mean 32 g
- Lipid content: 5 %
- Feeding during test (no diet on day of sampling):
- Food type: pelleted feed for carp, Japan Haigo Shiryo K.K.
- Amount: corresponding to 2 % of bw
- Frequency: 2x/d


ACCLIMATION
- Acclimation period: 1 - 2 months after external disinfection
- Disinfection: for about 24 h under static condition using 50 mg/L Terramycin and 7 g/L NaCl
- Acclimation conditions (same as test or not): flow-through, 25 +-2°C
- Health during acclimation (any mortality observed): abnormal fish removed

Study design

Route of exposure:

aqueous

Test type:

flow-through

Water / sediment media type:

natural water: freshwater

Total exposure / uptake duration:

8 wk

Test conditions

Test temperature:

25 +-2°C

Dissolved oxygen:

6 - 8 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: glass tank, 100 L test volume
- Type of flow-through (e.g. peristaltic or proportional diluter):
- Renewal rate of test solution (frequency/flow rate): 1155 L/d = approx. 0.8 L/min
- No. of organisms per vessel: 8 - 10 fish per test concentration
- No. of vessels per concentration (replicates): 1
- Biomass loading rate: =< 3 g/L (initial)
- Test period: 8 weeks


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: underground water


OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: no data

Nominal and measured concentrations:

1.5 and 15 µg/L

Reference substance (positive control):

no

Details on estimation of bioconcentration:

BASIS FOR CALCULATION OF BCF

BCF = conc(total fish tissue, wet) / conc(water)

Results and discussion

Bioaccumulation factor

Bioaccumulation factor 1

Type:

BCF

Value:

2 615

Basis:

whole body w.w.

Remarks:

average of two samples

Time of plateau:

6 wk

Calculation basis:

steady state

Remarks on result:

other: Conc.in environment / dose:15 µg/L

Bioaccumulation factor 2

Type:

BCF

Value:

2 105

Basis:

whole body w.w.

Remarks:

average of two samples

Time of plateau:

8 wk

Calculation basis:

steady state

Remarks on result:

other: Conc.in environment / dose:15 µg/L

Bioaccumulation factor 3

Type:

BCF

Value:

1 990

Basis:

whole body w.w.

Remarks:

one sample

Time of plateau:

6 wk

Calculation basis:

steady state

Remarks on result:

other: Conc.in environment / dose:1.5 µg/L

Bioaccumulation factor 4

Type:

BCF

Value:

1 790

Basis:

whole body w.w.

Remarks:

average of two samples

Time of plateau:

8 wk

Calculation basis:

steady state

Remarks on result:

other: Conc.in environment / dose:1.5 µg/L

Depuration

Elimination:

not specified

Details on results:

- Mortality of test organisms: none
- Behavioural abnormalities: none
- Other biological observations: none

Any other information on results incl. tables

Measured concentration in test water [µg/L]:

Level [µg/L]	2w	4w	6w	8w
15	14.8	15.6	15.4	15.4
1.5	1.56	1.53	1.50	1.51

Bioconcentration factor (based on measured concentration):

Level [µ/L]	2w	4w	6w	8w
15	1840	1920	2410	2750
	2060	2340	2820	1660
Mean	1950	2130	2615	2105
1.5	2110	1790	1990	1880
	2020	2710	903	1700
Mean	2065	2250	?	1790