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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Environmental fate & pathways

Bioaccumulation: aquatic / sediment

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Administrative data

Endpoint:
bioaccumulation in aquatic species: fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study with acceptable restrictions

Data source

Referenceopen allclose all

Reference Type:
publication
Title:
Unnamed
Year:
1992
Reference Type:
secondary source
Title:
European Union Risk Assessment Report, Nitrobenzene, CAS No: 98-95-3
Author:
European Chemicals Bureau
Year:
2007
Bibliographic source:
3rd Priority List, Volume: 77

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 305 C (Bioaccumulation: Test for the Degree of Bioconcentration in Fish)
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Nitrobenzene
EC Number:
202-716-0
EC Name:
Nitrobenzene
Cas Number:
98-95-3
Molecular formula:
C6H5NO2
IUPAC Name:
nitrobenzene

Test solutions

Vehicle:
not specified

Test organisms

Test organisms (species):
Cyprinus carpio
Details on test organisms:
TEST ORGANISM
- Common name: Carp
- Source: Sugishima fish farm, Japan
- Length at study initiation (lenght definition, mean, range and SD): ca. 10 cm
- Weight at study initiation (mean and range, SD): ca. 30 g
- Method of breeding: Pelleted feed for carp

ACCLIMATION
- Acclimation period: 28 days
- Acclimation conditions (same as test or not): 25 °C +/- 1 °C

Study design

Route of exposure:
aqueous
Test type:
flow-through
Water / sediment media type:
natural water: freshwater
Total exposure / uptake duration:
42 d

Test conditions

Test temperature:
25 °C
Details on test conditions:
- Test vessel:
- Material, size, headspace, fill volume: glass tanks of 100 liters in volume
- Type of flow-through (e.g. peristaltic or proportional diluter): Aquatron
- Renewal rate of test solution (frequency/flow rate): 200-800 ml/min
- No. of organisms per vessel: 15 - 20
- Source/preparation of dilution water: Dilution water used for the test originated from underground water pumped up from the ground of Kurume Research Laboratories.
- Intervals of water quality measurement: twice a week

TEST SYSTEM
Details on test conditions:
- Test vessel:
- Material, size, headspace, fill volume: glass tanks of 100 liters in volume
- Type of flow-through (e.g. peristaltic or proportional diluter): Aquatron
- Renewal rate of test solution (frequency/flow rate): 200-800 ml/min
- No. of organisms per vessel: 15 - 20

Test medium/ water parameters
- Source/preparation of dilution water: Dilution water used for the test originated from underground water pumped up from the ground of Kurume Research Laboratories.
- Intervals of water quality measurement: twice a week
Nominal and measured concentrations:
0.125 mg/l
0.0125 mg/l

Results and discussion

Bioaccumulation factoropen allclose all
Type:
BCF
Value:
ca. 3.1 - ca. 4.8
Basis:
other: Concentration of test substance in fish, no further data available.
Remarks on result:
other: Conc.in environment / dose:0.125 mg/L
Type:
BCF
Value:
ca. 1.6 - ca. 7.7
Basis:
other: Concentration of test substance in fish, no further data available.
Remarks on result:
other: Conc.in environment / dose:0.0125 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:
yes