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Diss Factsheets

Administrative data

Description of key information

Decahydronaphthalene has a low acute toxicity by the oral and dermal route with an oral LD50 (rat) of 4170 mg/kg bw (Smyth et al., 1951) and a dermal LD50 (rat) of 5200 mg/kg bw (Smyth et al., 1951).

Upon inhalation exposure the LC50 was 4.08 mg/L air (MacEwen, Vernot, 1978).

Key value for chemical safety assessment

Acute toxicity: via oral route

Link to relevant study records
Reference
Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1949-1951
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study briefly summarised, meets generally accepted scientific principles, acceptable for assessment. Restrictions: no guideline, no GLP
Principles of method if other than guideline:
Smyth HF Jr, Carpenter CP (1944): The place of the range finding test in the industrial toxicology laboratory. J. Ind. Hyg. Toxicol. 26, 269-273 and subsequent updates
GLP compliance:
no
Test type:
standard acute method
Limit test:
no
Species:
rat
Strain:
Sherman
Sex:
male
Route of administration:
oral: gavage
Vehicle:
water
No. of animals per sex per dose:
10
Control animals:
not specified
Details on study design:
14 days observation period
Statistics:
Thompson, W.R. (1947): Use of moving averages and interpolation to estimate median effective dose. Bact. Rev. 11, 115
Key result
Sex:
male
Dose descriptor:
LD50
Effect level:
4 170 mg/kg bw
Based on:
test mat.
Remarks on result:
other: (3.36-5.16 g/kg bw)
Mortality:
LD50 = 4170 ((3.36-5.16 g/kg bw) mg/kg bw
Clinical signs:
other:
Conclusions:
The LD50 value (oral) of decahydronaphthalene in male rats was estimated to be 4170 mg/kg bw based on a 14 day observation period. Thus the test substance showed a low toxicity in rats after oral gavage.
Executive summary:

The LD50 value (oral) of decahydronaphthalene in male rats was estimated to be 4170 mg/kg bw based on a 14 day observation period. Thus the test substance showed a low toxicity in rats after oral gavage.

Endpoint conclusion
Endpoint conclusion:
no adverse effect observed
Dose descriptor:
LD50
Value:
4 170 mg/kg bw
Quality of whole database:
Klimisch 2 (reliable with restrictions)

Acute toxicity: via inhalation route

Link to relevant study records
Reference
Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1977-06 to 1978-05
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Study well documented, sufficient information about test material, test organisms
Principles of method if other than guideline:
Acute inhalation toxicity similar to OECD 403
GLP compliance:
not specified
Test type:
standard acute method
Limit test:
no
Species:
rat
Strain:
Sprague-Dawley
Sex:
male
Details on test animals or test system and environmental conditions:
TEST ANIMALS:
Species/ Strain: rats, Sprague-Dawley
Sex: male
Source: Harlan Industries Inc.
Route of administration:
inhalation: vapour
Type of inhalation exposure:
whole body
Vehicle:
not specified
Details on inhalation exposure:
9 l glass chamber
Analytical verification of test atmosphere concentrations:
yes
Remarks:
hydrocarbon analyser
Duration of exposure:
4 h
Concentrations:
980, 820, 785, 625, 375 ppm
No. of animals per sex per dose:
5
Control animals:
no
Details on study design:
- exposure to measured concentrations of the test substance to determine an 4 hour LC 50
- Duration of observation period following administration: 14 days
Statistics:
Finney's probit analysis
Preliminary study:
Exposure of 5 male rats each to saturated vapours for 4, 2 and 1-hour resulted in death of 5, 5 and 2 rats respectively. The rats were hyperactive early in the exposure but after approximately 40 minutes exhibited tonic convulsions, tremors and prostration. Rats that survived two hours or more of exposure were paralysed in the posterior half of the body and this paralysis persisted for several days. None of the paralysed rats survived the 14-day postexposure observation period.
Key result
Sex:
male
Dose descriptor:
LC50
Effect level:
710 ppm
Based on:
test mat.
95% CL:
> 619 - < 816
Exp. duration:
4 h
Key result
Sex:
male
Dose descriptor:
LC50
Effect level:
4.08 mg/L air
Based on:
test mat.
Exp. duration:
4 h
Remarks on result:
other: calculated
Mortality:
All 5 animals died at 980 ppm, dose dependend decrease of mortality with non mortalities at 375 ppm
Clinical signs:
other: Animals displayed tremour and convulsions followed by prostration. Most survivours displayed hind quarter paralysis
Gross pathology:
Mild to severe congestion of the lungs with occasional areas of atelectasis was noted in animals that died. Reticulation of the liver and pale coloration of most organs was a common finding at all concentration levels.

 




































Concentration ppm



Concentration mg/L



Mortality



980



5.55



5/5



820



4.65



4/5



785



4.45



2/5



625



3.54



2/5



375



2.12



0/5


Conclusions:
LC50 was determiend at 710 ppm ( with a 95% confidence intervall of 619-816 ppm). This corresponds to 4.08 mg/L air.
Executive summary:

Male Sprague-Dawley rate (5 per group) a were exposed for 4 hours to varying concentrations of Decalin vapor (375 -980 ppm). Animals were exposed in a 9-liter glas chamber and observed for 14-days post exposure. Chamber concentrations were analyzed using a hydrocarbon analyzer. LC50 was calculated using Finney's probit analysis method. Reticulation of the liver and pale coloration of most organs was a common finding at all concentration levels. 


Animals displayed tremors and convulsions followed by prostration. Most survivors displayed hind quarter paralysis. Gross pathologic examination of animals that died showed mild to severe congestion of the lungs with occasional areas of atelectasis.

Endpoint conclusion
Endpoint conclusion:
adverse effect observed
Dose descriptor:
LC50
Value:
4 080 mg/m³ air
Quality of whole database:
Klimisch 1 (reliable without restrictions)

Acute toxicity: via dermal route

Link to relevant study records
Reference
Endpoint:
acute toxicity: dermal
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1949-1951
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study briefly summarised, meets generally accepted scientific principles, acceptable for assessment. Restrictions: Only male animals used, no guideline, no GLP
Principles of method if other than guideline:
Smyth HF Jr, Carpenter CP (1944): The place of the range finding test in the industrial toxicology laboratory. J. Ind. Hyg. Toxicol. 26, 269-273 and subsequent updates
GLP compliance:
no
Test type:
standard acute method
Limit test:
no
Species:
rabbit
Strain:
not specified
Sex:
male
Type of coverage:
semiocclusive
Vehicle:
unchanged (no vehicle)
Details on dermal exposure:
- Area covered: clipped trunk
- Occlusion: impervious flexible film ("vinylite" sheeting)
- Exposure period: 24 hours
- Observation period: 14 days
Duration of exposure:
24 h
Doses:
no data
No. of animals per sex per dose:
5
Control animals:
no
Statistics:
Thompson, W.R. (1947): Use of moving averages and interpolation to estimate median effective dose. Bact. Rev. 11, 115
Key result
Sex:
male
Dose descriptor:
LD50
Effect level:
5 200 mg/kg bw
95% CL:
3 380 - 8 000
Mortality:
LD50 = 5.9 (3.84-9.06) mL/kg bw
=5200 (3380-8000) mg/kg bw
Clinical signs:
other:
Conclusions:
The LD50 (rabbit) for decahydronaphthalene applied to the skin of the rabbit is 5.9 mL/kg bw (=5.2 g/kg bw). Thus decahydronaphthalene showed low acute toxicity in rabbits after dermal application.
Executive summary:

The LD50 for decahydronaphthalene applied undiluted to the clipped skin of the rabbit trunk under "Vinylit" sheeting for 24h was 5.9 mL/kg bw (95% confidence limit 3.84-9.06). This corresponded to 5200 (3380-8000) mg/kg bw.

Endpoint conclusion
Endpoint conclusion:
no adverse effect observed
Dose descriptor:
LD50
Value:
5 200 mg/kg bw
Quality of whole database:
Klimisch 2 (reliable with restrictions)

Additional information

Justification for classification or non-classification

Decahydronaphthalene has a low acute toxicity by the oral and dermal route, the experimental evidence indicates that limit values are exceeded and therefore no classification is required for acute oral and dermal toxicity according to CLP regulation 1272/2008.


Upon inhalation exposure the LC50 was 4.08 mg/L air. Thus, decahydronaphthalene has to be classified for acute inhalation toxicity according CLP regulation 1272/2008, Annex I (Category 3, H301: Toxic if inhaled).