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EC number: 700-580-1
CAS number: -
Nominal test substanceconcentration
Total number of dead fish after the start of exposure
A limit-test with a
concentration of nominal 100 mg test substance per L dilution water was
7 Zebrafish(Danio rerio)
were exposed in the test substance group and 7 in the control group.
Their length was between 3.1 and 3.5 cm.
50 % solution of test substance in water was used. According to the
sponsor the actual concentration of the test substance was 48.5 % w/w. A
nominal test substance concentration of 100 mg/L was therefore prepared
by dissolving 1031mg test substance in 5 litres dilution water and manual
homogenization. The dilution water was local tap water, mixed 1:1 with
deionised water, to achieve the appropriate water hardness as required
by the guideline.
This dilution water was also
used as the negative control substance and for fish holding prior the
The exposure of the Zebrafish
was for 96 h in a semi-static test with renewal of test and control
media after 24, 48 and 72 hours.
The results of the study revealed a
LC50 after 96 h of exposure of more than 100 mg/L, a NOEC of 100 mg/L
and a LC0 after 96 hours greater or equal 100 mg/L.
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